LOCUS pIEx/Bac-2-3CLIC 6805 bp DNA circular 17-OCT-2006
DEFINITION pIEx/BAC/p10 Strep/3cLIC/Tb/10xHis.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_recomb complement(2886..4304)
/dnas_title="1629"
/vntifkey=86
/label=1629
rep_origin 6241..6696
/dnas_title="f1 origin"
/vntifkey=33
/label=f1 origin
CDS complement(5252..6109)
/dnas_title="Ap"
/vntifkey=4
/label=Ap
rep_origin complement(4491..4491)
/dnas_title="origin"
/vntifkey=33
/label=origin
misc_recomb 4..453
/dnas_title="lef-2"
/vntifkey=86
/label=lef-2
misc_recomb complement(491..1096)
/dnas_title="ORF 603"
/vntifkey=86
/label=ORF 603
rep_origin 4324..5083
/dnas_title="pUC Ori"
/vntifkey=33
/label=pUC Ori
enhancer 1196..1677
/dnas_title="hr5"
/vntifkey=9
/label=hr5
polyA_signal 2712..2717
/dnas_title="PolyA signal"
/vntifkey=25
/label=PolyA signal
5'UTR 2229..2278
/dnas_title="ie1 5'-UTR"
/vntifkey=52
/label=ie1 5'-UTR
promoter 2279..2391
/dnas_title="p10 promoter + 5'UTR"
/vntifkey=29
/label=p10 promoter + 5'UTR
promoter 1682..2228
/dnas_title="ie1 promoter"
/vntifkey=29
/label=ie1 promoter
terminator 2535..2841
/dnas_title="ie1 Terminator"
/vntifkey=43
/label=ie1 Terminator
protein_bind 2403..2426
/dnas_title="Strep-Tag II"
/vntifkey=31
/label=Strep-Tag II
protein_bind 2433..2455
/dnas_title="3c"
/vntifkey=31
/label=3c
misc_binding 2487..2516
/dnas_title="10x His-Tag"
/vntifkey=20
/label=10x His-Tag
protein_bind 2463..2480
/dnas_title="Thrombin"
/vntifkey=31
/label=Thrombin
misc_feature 2458..2459
/note="the joint between these two bases would result if 3CLIC ends were filled in and ligated"
BASE COUNT 1814 a 1453 c 1497 g 2002 t
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcaa
2401 gctggagcca cccgcagttc gaaaagggtg cacttgaagt cctctttcag ggacccggcg
2461 ctctggtgcc acgcggtagt tccgctcatc accaccatca tcaccatcac caccactaag
2521 tgattaacct caggttatac atatattttg aatttaatta attatacata tattttatat
2581 tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca tagaaataac
2641 aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt tcatcatgta
2701 tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca acaaaatgat
2761 aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga ctaaaccttc
2821 atcgcccagt aaccaatcta ggtagctgag cgcatgcaag ctgatccggg ttattagtac
2881 atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt
2941 aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt
3001 tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt
3061 cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt
3121 tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc
3181 gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta
3241 tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaatagagct
3301 tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa
3361 ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg acgcctatta
3421 attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg aattatttct
3481 gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa ttcagacaac
3541 acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc tactaatggc
3601 ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg ggctggcggc
3661 ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc aaatgtctct
3721 ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt ttttggtttg
3781 accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa ttgttgtctg
3841 tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg cggcaattca
3901 gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg agaaggtggt
3961 ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac gattgtgggc
4021 accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg cagcgcttgg
4081 ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc tataagcatt
4141 gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta agcaattgta
4201 ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa gccttttcat
4261 ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgaggttta aacgcttcct
4321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4921 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
5041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
5101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
6121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6241 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
6301 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
6361 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
6421 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
6481 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
6541 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
6601 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
6661 acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca
6721 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca
//