LOCUS pIEx/Bac-3-3CLIC 6802 bp DNA circular 17-OCT-2006
DEFINITION pIEx/BAC/p10 10xHis/3cLIC/Tb/Strep.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
misc_recomb complement(2883..4301)
/dnas_title="1629"
/vntifkey=86
/label=1629
rep_origin 6238..6693
/dnas_title="f1 origin"
/vntifkey=33
/label=f1 origin
CDS complement(5249..6106)
/dnas_title="Ap"
/vntifkey=4
/label=Ap
rep_origin complement(4488..4488)
/dnas_title="origin"
/vntifkey=33
/label=origin
misc_recomb 4..453
/dnas_title="lef-2"
/vntifkey=86
/label=lef-2
misc_recomb complement(491..1096)
/dnas_title="ORF 603"
/vntifkey=86
/label=ORF 603
rep_origin 4321..5080
/dnas_title="pUC Ori"
/vntifkey=33
/label=pUC Ori
enhancer 1196..1677
/dnas_title="hr5"
/vntifkey=9
/label=hr5
polyA_signal 2709..2714
/dnas_title="PolyA signal"
/vntifkey=25
/label=PolyA signal
5'UTR 2229..2278
/dnas_title="ie1 5'-UTR"
/vntifkey=52
/label=ie1 5'-UTR
promoter 2279..2391
/dnas_title="p10 promoter + 5'UTR"
/vntifkey=29
/label=p10 promoter + 5'UTR
promoter 1682..2228
/dnas_title="ie1 promoter"
/vntifkey=29
/label=ie1 promoter
terminator 2532..2838
/dnas_title="ie1 Terminator"
/vntifkey=43
/label=ie1 Terminator
protein_bind 2490..2513
/dnas_title="Strep-Tag II"
/vntifkey=31
/label=Strep-Tag II
protein_bind 2436..2458
/dnas_title="3c"
/vntifkey=31
/label=3c
misc_feature 2466..2482
/dnas_title="Thrombin site"
/vntifkey=21
/label=Thrombin site
misc_binding 2400..2429
/dnas_title="10x His-Tag"
/vntifkey=20
/label=10x His-Tag
misc_feature 2461..2462
/note="the joint between these two bases is the 3C/LIC
junction if ends were filled in and ligated."
BASE COUNT 1813 a 1452 c 1496 g 2002 t
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tccatggcac
2401 atcaccacca tcatcaccat caccaccacg gtgcacttga agtcctcttt cagggacccg
2461 gcgctctggt gccacgcggt agttccgctt ggagccaccc gcagttcgaa aagtaagtga
2521 ttaacctcag gttatacata tattttgaat ttaattaatt atacatatat tttatattat
2581 ttttgtcttt tattatcgag gggccgttgt tggtgtgggg ttttgcatag aaataacaat
2641 gggagttggc gacgttgctg cgccaacacc acctcccttc cctcctttca tcatgtatct
2701 gtagataaaa taaaatatta aacctaaaaa caagaccgcg cctatcaaca aaatgatagg
2761 cattaacttg ccgctgacgc tgtcactaac gttggacgat ttgccgacta aaccttcatc
2821 gcccagtaac caatctaggt agctgagcgc atgcaagctg atccgggtta ttagtacatt
2881 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
2941 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
3001 gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga
3061 ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg
3121 ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt
3181 tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt
3241 ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa tagagcttgg
3301 acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc
3361 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
3421 gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat
3481 tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg
3541 ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc
3601 ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga
3661 ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta
3721 ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc
3781 ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg
3841 tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac
3901 atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc
3961 ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc
4021 ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt
4081 ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta
4141 atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg
4201 taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt
4261 tactacagca ttgtagtggc gagacacttc gctgtcgtcg aggtttaaac gcttcctcgc
4321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4921 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
5281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
5341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
5401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
5461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
5521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
5581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
5641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
5701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
5761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
5821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
5881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
5941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
6001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
6061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
6121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
6181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
6241 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
6301 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
6361 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
6421 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
6481 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
6541 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
6601 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
6661 cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact
6721 gttgggaagg gcgatcggtg cgggcctctt cgctattacg cca
//