LOCUS pIEx/Bac-4-EkLIC 7454 bp DNA circular 14-NOV-2007
DEFINITION pIEx/Bac-4 EkLIC p10/AAT/Pseudo UTR/GST/EkLIC/10xHis.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
promoter 1682..2228
/vntifkey="29"
/label=ie1\Promoter
misc_recomb complement(3574..4992)
/vntifkey="86"
/label=1629
rep_origin 6929..7384
/vntifkey="33"
/label=f1\origin
CDS complement(5940..6797)
/vntifkey="4"
/label=Ap
rep_origin complement(5179..5179)
/vntifkey="33"
/label=origin
misc_recomb 4..453
/vntifkey="86"
/label=lef-2
misc_recomb complement(491..1096)
/vntifkey="86"
/label=ORF\603
rep_origin 5012..5771
/vntifkey="33"
/label=pUC\Ori
enhancer 1196..1677
/vntifkey="9"
/label=hr5
polyA_signal 3400..3405
/vntifkey="25"
/label=PolyA\signal
misc_feature 3368..3371
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
misc_feature 3137..3151
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
misc_binding 3161..3190
/vntifkey="20"
/label=10x\His-Tag
primer 3155..3203
/vntifkey="27"
/label=S-CTerm\10xHis
terminator 3223..3529
/vntifkey="43"
/label=ie1\Terminator
primer 3207..3220
/vntifkey="27"
/label=AS-Bsu36\I\Adapt\Avr
5'UTR 2229..2278
/vntifkey="52"
/label=ie1\5'-UTR
promoter 2279..2392
/vntifkey="29"
/label=p10\promoter\+\5'UTR
misc_feature 2277..2280
/vntifkey="21"
/label=Bsa\I\jct
CDS 2435..3094
/invitrogen="1170000"
/vntifkey="4"
/label=GST\tag\(no\stop)
misc_feature 2395..2434
/vntifkey="21"
/label=polH\pseudo\5'-UTR\ATT
protein_bind 3119..3133
/vntifkey="31"
/label=Ek\site
primer 2216..2237
/vntifkey="27"
/label=IE1\Promoter\Primer
primer 2325..2345
/vntifkey="27"
/label=p10\Promoter\primer
primer complement(3285..3303)
/vntifkey="27"
/label=IE1\Terminator\Primer
misc_feature 3122..3133
/vntifkey="21"
/label=Bottom\strand\single\stranded\for\EkLIC
misc_feature 3137..3150
/vntifkey="21"
/label=Upper\strand\single\stranded\for\EkLIC
misc_feature 3134..3136
/vntifkey="21"
/label=Delete\for\EkLIC
BASE COUNT 2022 a 1576 c 1639 g 2214 t 3 others
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga
2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa
2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcaga
3121 tgacgacgac aagnnnccgg gcttctcctc aatttccgct catcaccacc atcatcacca
3181 tcaccaccac taagtgatta acctcacgac ctaggcctca ggttatacat atattttgaa
3241 tttaattaat tatacatata ttttatatta tttttgtctt ttattatcga ggggccgttg
3301 ttggtgtggg gttttgcata gaaataacaa tgggagttgg cgacgttgct gcgccaacac
3361 cacctccctt ccctcctttc atcatgtatc tgtagataaa ataaaatatt aaacctaaaa
3421 acaagaccgc gcctatcaac aaaatgatag gcattaactt gccgctgacg ctgtcactaa
3481 cgttggacga tttgccgact aaaccttcat cgcccagtaa ccaatctagg tagctgagcg
3541 catgcaagct gatccgggtt attagtacat ttattaagcg ctagattctg tgcgttgttg
3601 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
3661 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
3721 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
3781 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
3841 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
3901 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
3961 actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt
4021 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
4081 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
4141 tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct
4201 aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa
4261 catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat
4321 cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc
4381 agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt
4441 actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt
4501 tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt
4561 cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc
4621 tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg
4681 tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga
4741 aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata
4801 caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt
4861 taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tcggatcgat
4921 cccgcacgcc gataacaagc cttttcattt ttactacagc attgtagtgg cgagacactt
4981 cgctgtcgtc gaggtttaaa cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
5041 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
5101 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
5161 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
5221 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
5281 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
5341 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
5401 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
5461 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
5521 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
5581 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
5641 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
5701 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
5761 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
5821 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
5881 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
5941 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
6001 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
6061 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
6121 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6181 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6241 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6301 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
6361 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
6421 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
6481 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
6541 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
6601 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
6661 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
6721 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
6781 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
6841 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
6901 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
6961 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
7021 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
7081 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
7141 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
7201 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
7261 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
7321 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
7381 atttcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
7441 tcgctattac gcca
//