LOCUS pIEx/Bac-5-3CLIC 7463 bp DNA circular 14-NOV-2007
DEFINITION pIEx/Bac-5 3CLIC p10/AAT/Pseudo UTR/GST/3CLIC/Strep.
SOURCE
ORGANISM
FEATURES Location/Qualifiers
promoter 1682..2228
/vntifkey="29"
/label=ie1\Promoter
misc_recomb complement(3583..5001)
/vntifkey="86"
/label=1629
rep_origin 6938..7393
/vntifkey="33"
/label=f1\origin
CDS complement(5949..6806)
/vntifkey="4"
/label=Ap
rep_origin complement(5188..5188)
/vntifkey="33"
/label=origin
misc_recomb 4..453
/vntifkey="86"
/label=lef-2
misc_recomb complement(491..1096)
/vntifkey="86"
/label=ORF\603
rep_origin 5021..5780
/vntifkey="33"
/label=pUC\Ori
enhancer 1196..1677
/vntifkey="9"
/label=hr5
polyA_signal 3409..3414
/vntifkey="25"
/label=PolyA\signal
misc_feature 3377..3380
/vntifkey="21"
/label=3x\-BseR\I\TCCT\to\CTTC
terminator 3232..3538
/vntifkey="43"
/label=ie1\Terminator
protein_bind 3176..3199
/vntifkey="31"
/label=Strep-Tag\II
misc_feature 3152..3168
/vntifkey="21"
/label=Thrombin\site
primer 3170..3212
/vntifkey="27"
/label=S-CTerm\Strep
primer 3216..3229
/vntifkey="27"
/label=AS-Bsu36\I\Adapt\Avr
5'UTR 2229..2278
/vntifkey="52"
/label=ie1\5'-UTR
promoter 2279..2392
/vntifkey="29"
/label=p10\promoter\+\5'UTR
misc_feature 2277..2280
/vntifkey="21"
/label=Bsa\I\jct
CDS 2435..3094
/invitrogen="1170000"
/vntifkey="4"
/label=GST\tag\(no\stop)
misc_feature 2395..2434
/vntifkey="21"
/label=polH\pseudo\5'-UTR\ATT
protein_bind 3119..3141
/vntifkey="31"
/label=3c
primer 2216..2237
/vntifkey="27"
/label=IE1\Promoter\Primer
primer complement(3294..3312)
/vntifkey="27"
/label=IE1\Terminator\Primer
misc_feature 3134..3144
/vntifkey="21"
/label=Bottom\Strand\single\stranded\for\3C/LIC
misc_feature 3148..3159
/vntifkey="21"
/label=Top\strand\single\stranded\for\3C/LIC
misc_feature 3145..3147
/vntifkey="21"
/label=Delete\for\3C/LIC
BASE COUNT 2017 a 1572 c 1653 g 2218 t 3 others
ORIGIN
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt
1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg
1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt
1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag
1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata
1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta
1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag
1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga
1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg
1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg
1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc
1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa
1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg
1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg
1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc
2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga
2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata
2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat
2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc
2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga
2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa
2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcact
3121 tgaagtcctc tttcagggac ccggnnncgc tctggtgcca cgcggtagtt ccgcttggag
3181 ccacccgcag ttcgaaaagt aagtgattaa cctcacgacc taggcctcag gttatacata
3241 tattttgaat ttaattaatt atacatatat tttatattat ttttgtcttt tattatcgag
3301 gggccgttgt tggtgtgggg ttttgcatag aaataacaat gggagttggc gacgttgctg
3361 cgccaacacc acctcccttc cctcctttca tcatgtatct gtagataaaa taaaatatta
3421 aacctaaaaa caagaccgcg cctatcaaca aaatgatagg cattaacttg ccgctgacgc
3481 tgtcactaac gttggacgat ttgccgacta aaccttcatc gcccagtaac caatctaggt
3541 agctgagcgc atgcaagctg atccgggtta ttagtacatt tattaagcgc tagattctgt
3601 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
3661 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
3721 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
3781 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
3841 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
3901 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
3961 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
4021 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
4081 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
4141 gatcaaattt gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg
4201 tttcaatcta actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg
4261 cggtggtaac atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa
4321 atctaccatc ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg
4381 cggtgatgca gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc
4441 aactattgta ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt
4501 tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg
4561 aggttccgtc ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg
4621 tggaggcgct ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat
4681 ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg
4741 cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata
4801 attggaatac aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt
4861 gccgatattt aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct
4921 cggatcgatc ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc
4981 gagacacttc gctgtcgtcg aggtttaaac gcttcctcgc tcactgactc gctgcgctcg
5041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
5101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
5161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
5221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
5281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
5341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
5461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
5581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
5641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
5701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
5821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
6001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
6061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
6181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
6241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
6301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
6361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
6421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
6481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
6541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
6601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
6661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
6721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
6781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
6901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
6961 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
7021 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
7081 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
7141 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
7201 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
7261 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
7321 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
7381 acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
7441 cgggcctctt cgctattacg cca
//