Pseudoproline dipeptides are extremely simple to use. They are introduced into the peptide sequence using standard coupling methods, substituting any Aaa-Ser or Aaa-Thr dipeptide.

Experience has led to the development of the following empirical guidelines for their use:

• Optimal results are obtained if the PPs are spaced 5-6 residues apart throughout the sequence.
• The optimum separation between a PP and a Pro residue is 5-6 amino acid residues.
• The minimum separation between a PP and another PP or Pro residue is 2 residues.
• Aim to insert a PP before regions of hydrophobic residues.