KOD DNA Polymerase* ^† (formerly KOD DNA HiFi Polymerase) is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase (1). KOD is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield (2). The enzyme’s 3´→5´exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA polymerase, resulting in highly accurate and robust yields in short reaction times. The enzyme generates blunt-ended PCR products suitable for cloning with the Novagen^® Perfectly Blunt^® and LIC Vector Kits.

Features and Benefits:
	Higher fidelity than Pfu DNA polymerase—excellent for cloning
	Greater yield—extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
	More accurate PCR in a shorter time
	Higher processivity—sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
	Does not result in truncated amplification products

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* Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

† Manufactured by TOYOBO and distributed by EMD Chemicals. Not available from Merck Chemicals in Japan.