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New KOD Xtreme™ Hot Start DNA Polymerase
 |   KOD DNA Polymerases Overview | KOD Hot Start DNA Polymerase | | KOD XL DNA Polyerase | | KOD Citations by Application | | |
The KOD Xtreme™ Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kb respectively. It successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer, and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen® Perfectly Blung® and LIC Vector Kits.
Features and Benefits: - Optimized for the highest PCR success rate, even with the most difficult targets
- Efficiently amplifies up to 90% GC-content templates
- 10X higher fidelity than Taq blends
- Amplifies genomic targets up to 24 kb
- Amplifies plasmid/lambda targets up to 40 kb
- Elliminates mispriming and primer-dimer formation
- Convenient ambient-temperature setup compatible with automation
Amplification of Difficult DNA Targets Using KOD Xtreme Hot Start DNA Polymerase Challenging PCR targets pose unique problems that may be difficult to address using standard polymerase systems. KOD Xtreme Hot Start DNA Polymerase successfully amplified numerous targets, including up to 40 kb long and up to 90% GC-rich products, from a variety of templates. The accuracy of KOD Xtreme Hot Start DNA Polymerase is 10X that Taq blends traditionally used for these applications, as it utilizes high-fidleity KOD DNA Polymerase (Raul 2004, Nishioka 2001), Takagi 1997). The compatibility of KOD Xtreme polymerase with hot start cycling programs is an advantage for both low- and high-throughput applications. 
Performance of KOD Xtreme Hot Start DNA Polymerase versus competitors' polymerase in amplification of genomic DNA
A 6273 bp region of the GAD67 target was amplified from 100 ng chick kidney genomic DNA. PCR cycling parameters for KOD Xtreme were as follows: initial denaturation at 94°C for 2 min; 35 cycles at 98°C for 10 s, 68°C for 7 min. For polymerases from other manufacturers, optimal cycling parameters as recommended by each manufacturer were used. These data were provided by a member of the Faculty of Medicine, Kyoto University. For more detail on these and other experiments click to see the full newsletter article.
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M | 1kb Ladder Marker |
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1,2 | Products of KOD Xtreme Hot Start DNA Polymerase |
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3,4 | Products of Ex Tag™ Polymerase |
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5,6 | Products of LA Tag™ Polymerase |
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| Performance of KOD Xtreme Hot Start DNA Polymerase versus competitors' polymerase in
amplification of genomic DNA from mouse tail lysates A 2.6 kb region of the Mouse membrane glycoprotein (Thy-1) gene <M10246> was amplified from
0.5 µl of crude mouse tail lysates (prepared using an alkaline lysis method). PCR cycling parameters for
KOD Xtreme were as follows: initial denatured at 94° C for 2 min, 30 cycles at 98° C for 10 s, 68° C
for 2.5 min.
For polymerases from other manufacturers, optimal cycling parameters as
recommended by each manufacturer were used.
Lane(s) | Samples |
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M | 1kb Ladder Marker |
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1,2,3 | Products of KOD Xtreme Hot Start DNA Polymerase amplified from 1,2, or 4 µl |
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4,5,6 | Product of PfuTurbo® DNA Polymerase amplified from 1, 2 or 4 µl of whole blood |
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7,8,9 | Products of Ex Taq Polymerase amplified from 1, 2, or 4 µl of whole blood |
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10,11,12 | Products of La Taq Polymerase amplified from 1, 2, or 4 µl whole blood |
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13,14,15 | Products of Taq DNA Polymerase amplified from 1, 2, or 4 µl of whole blood |
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| Performance of KOD Xtreme Hot Start DNA Polymerase versus competitors' polymerase in amplification from whole blood samples
A 1.3 kb region of the b-globin gene was amplified from 1, 2 or 4 µl of untreated whole blood.
PCR cycling parameters for KOD Xtreme were as follows: initial denatured 94° C for
2 min; 30 cycles at 98° C for 10 s, 68° C for 1 min/kb. For polymerases from other manufacturers,
optimal cycling parameters as recommended by each manufacturer were used. Nishioka, M., et al. 2001. J. Biotechnol. 88, 141.
Raul, J.F., et al. 2004. Genome Res. 14, 2128 | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the
amount purchased for the purchaser's won internal research. Not other patents rights (such as 5' Nuclease Process patent rights) are
conveyed expressly, by implication or by estoppel. Further information on purchasing licenses may be obtained by contacting the
Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA, *Manufactured by TOYOBO and distributed by EMD Chemicals. Not available from Merck Chemicals in Japan. | | |
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