WideScreen™ Human Cancer Panel 2

WideScreen™ EpiTag™ Assays Overview Technology ERK Data Available Assays WideScreen™ RTK Assays RTK Assays RTK Data Available Assays WideScreen™ Biomarker Assays Human Cancer Panel 1 (Tumor Markers) Human Cancer Panel 2 (Growth Factors) Human CVD Panel 1 (Apolipoprotein) Human CVD Panel 2 (Inflammation) Human CVD Panel 3 Human CVD Panel 4 Human CVD Panel 5 (Acute Phase) Human CVD Panel 6

The WideScreen™ Human Cancer Panel 2 (Growth Factors) is a pre-mixed multiplex bead kit of quantitative antibody-based assays for simultaneous detection of twelve human growth factors in biological fluids: amphiregulin, betacellulin, EGF, EGFR, epiregulin, FGF-basic, HB-EGF, PDGF-BB, PlGF, tenascin C, TGF-a, and VEGF. The kit includes all the reagents and buffers needed to analyze the above proteins in serum, plasma, and tissue culture supernatants using the Luminex® xMAP® System.

Resources:
Sample Certificate of Analysis

Technical Bulletin:
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Product Cat. No. WideScreen™ Human Cancer Panel 1  NEW! 71984 WideScreen™ Human Cancer Panel 2  NEW! 71995

Select analyte measurements in normal and disease state serum samples: Normal (N, ,
n = 6), Colorectal cancer (CR, , n = 4), Ovarian cancer (OV, , n = 3), Gastrointestinal
cancer (GI, , n = 2). Data from two experiments are represented. Data points at or
below the corrected least detectable dose (LLD) are plotted on the x-axis. Serum samples
from normal individuals had clustered growth factor concentrations, whereas elevated
concentrations were found in some disease state patients.

Growth Factor Secretion by Human Cell Lines and PBMC
Cells were grown to confluence and stimulated as indicated. Supernatants were clarified
and diluted prior to testing. Targets that were below the assay level of detection are
denoted by (•). Results showing high level biomarker expression are consistent with
reports from the literature.