His•Tag Fusion Protein Detection Products  |
• His•Tag Monoclonal Antibody |
| • His•Tag Antibody Plate |
 Back to Purification His•Tag and GST•Tag Protein Purification and Detection |
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His•Tag Monoclonal Antibody
Sensitive, specific detection of His•Tag fusion proteins |
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The His•Tag Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10–8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1 to 0.2 μg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-Soluble Casein is recommended as a blocking agent. Please see Western Blot Kits & Reagents later in this chapter for a complete sets of reagents specifically configured for Western detection of the His•Tag Monoclonal Antibody.
The 100-μg package size provides enough purified antibody for 1000 ml of working solution.
* Not recommended for HRP-based detection of Trail Mix Western Markers.
| Product:
His•Tag Monoclonal Antibody
70796
Available seperately:
Goat Anti-Mouse IgG AP Conjugate (H+L)
69266-3
Goat Anti-Mouse HRP AP Conjugate (H+L)
71045-3
5% Alkali-Soluble Caesin
70955-3
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Immunohistochemical detection of His•Tag fusion proteins in transfected COS-1 cells
pTriEx™ plasmid DNA encoding a His•Tag firefly luciferase (Fluc) fusion protein was transiently transfected into COS-1 cells with GeneJuice® Transfection Reagent. Twenty-four hours after transfection, cells were fixed, blocked with BSA and horse serum, and then exposed to His•Tag Monoclonal Antibody (1:1000 dilution of 0.2 mg/ml) followed by a Cy3™-conjugated Goat Anti- Mouse IgG. Hoechst 33258 was used for visualization of cell nuclei. A, Immunofluorescent staining of His•Tag Fluc; B, Hoechst staining of the same field as in A showing both transfected and non-transfected cells. |
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His•Tag Antibody Plate
Immobilized antibody in 96-well format |
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 The His•Tag Antibody Plate is a 96-well, ELISA-compatible plate containing immobilized His•Tag Monoclonal Antibody. The antibody is covalently immobilized to the surface using a method that retains maximal binding activity. The antibody specifically recognizes five consecutive histidines, and so will bind with high affinity (Kd = 5 × 10–8– 1 × 10–9 M) to virtually any His•Tag fusion protein in which the tag is exposed. This plate has outstanding binding characteristics, with a capacity of > 100 ng His•Tag fusion protein per well and low non-specific binding. Well-to-well variability is less than 5% and the plates are stable when stored dry at 4°C. The His•Tag Antibody Plate can be used in a variety of binding assays where reliable, specific immobilization of His•Tag fusion proteins is required. | Product:
His•Tag Antibody Plate
71184
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