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GST•Tag Fusion Protein Detection Products  |
• GST•Tag™ Monoclonal Antibody | | • GST•Tag™ Antibody Plate | | • GST•Tag™ Assay Kit |  Back to Purification His•Tag and GST•Tag Protein Purification and Detection | | | GST•Tag Monoclonal Antibody
Sensitive, specific detection of GST•Tag fusion proteins | | The GST•Tag™ Monoclonal Antibody is a mouse monoclonal antibody (IgG1) with high affinity for the 26-kDa glutathione-S-transferase (GST) domain from Schistosoma japonicum (1–5). This highly purified antibody is superior for detection of fusion proteins containing the GST•Tag expressed with the pET–41 or pET–42 vector series or other GST-encoding vectors.
The 50-μg package size provides enough purified antibody to perform 50 Western blots (10 cm × 10 cm).
Please see the Western Blot Kits and Reagents for conjugates and substrates compatible with the determination of GST•Tag and other Novagen monoclonal and polyclonal antibodies.
1. Smith, D. B. and Johnson, K. S. (1988) Gene 67, 31–40.
2. Toye, B., Zhong, G. M., Peeling, R., and Brunham (1990) Infect. Immunol. 58, 3909–3913.
3. Fikrig, E. Barthold, S. W., Kantor, F. S., and Flavell, R. A.(1990) Science 250, 553–556.
4. Beekman, J. M., Cooney, A. J., Elliston, J. F., Tsai, S. Y., and Tsai, M. J. (1994) Gene 146, 285–289.
5. Poon, R. Y. and Hunt, T. (1994) Anal. Biochem. 218, 26–33. | Product:
GST•Tag™ Monoclonal Antibody
71097
Available seperately:
Goat Anti-Mouse IgG AP Conjugate (H+L)
69266-3
Goat Anti-Mouse HRP AP Conjugate (H+L)
71045-3
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Western blot detection of a GFP/GST•Tag fusion protein
Two parallel blots were incubated with GST•Tag Monoclonal Antibody, then incubated with Anti-Mouse IgG AP or HRP Conjugate and processed by colorimetric (left panel) or chemiluminescent (right panel) detection. | | |  |
Immunohistochemical detection of GST expressed in transfected COS-1 cells
A pTriEx™ vector expressing GST was transiently transfected into COS-1 cells with GeneJuice™ Transfection Reagent. Twentyfour hours after transfection, cells were fixed, blocked with BSA and horse serum, and then exposed to GST•Tag Monoclonal Antibody (1:10,000 dilution) followed by a Cy3™-conjugated Goat Anti-Mouse IgG. Hoechst 33258 was used for visualization of cell nuclei. A, Immunofluorescent staining of GST; B, Hoechst staining of the same field as in A showing both transfected and non-transfected cells. | | | GST•Tag Antibody Plate
Immobilized antibody in 96-well format | | |  The GST•Tag™ Antibody Plate is a 96-well, ELISA-compatible plate in an 8-well strip format containing immobilized GST•Tag Monoclonal Antibody, a mouse monoclonal antibody (IgG1) with high affinity for the 26-kDa glutathione-S-transferase (GST) domain of Schistosoma japonicum.
The antibody will bind with high affinity to virtually any GST•Tag fusion protein with an exposed tag. These plates have excellent binding characteristics, with a binding capacity of > 50 ng GST•Tag fusion protein per well and low non-specific binding. Well-to-well variability is less than 10%. The plates are stable when stored dry at 4°C. The GST•Tag Antibody Plate can be used in a variety of binding assays where reliable, specific immobilization of GST•Tag fusion proteins is required.
|  | | | Product:
GST•Tag™ Antibody Plate
71437
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| | | GST•Tag Assay Kit
Quantitative assay of GST•Tag fusion proteins | | | The GST•Tag™ Assay Kit is designed to perform quantitative colorimetric enzymatic assays of glutathione-S-transferase (GST) fusion proteins (1). The kit is useful for the quantification of GST activity in crude samples or purified fractions. The suitability of this assay for crude samples allows expression conditions to be evaluated and rapidly optimized by comparing GST activity levels. The GST activity assay is simple to perform using the supplied 1-chloro-2,4-dinitrobenzene (CDNB) substrate. A sample is combined with CDNB substrate in the supplied reaction buffer and the absorbance of the reaction is monitored at 340 nm. The rate of change in A340 is proportionate to the amount of GST activity present in the sample. The assay has sufficient sensitivity to detect as little as eight pmol of functional GST, which corresponds to approximately 250 ng of unfused GST.
1. Habig, W. H., Pabst, M. J., and Jakoby, W. B. (1974) J. Biol. Chem. 249, 7130–7139. | | | |
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