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Novagen inNovations Newsletter

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inNovations 29, September 2008
1. Full issue, 7271 KB
2. iFOLD� Protein Refolding System 3
3. WideScreen™ Human Cancer Panels 1 and 2
4. NusA Fusion System
5. plEX/Bac™ -4 and plEx/Bac-5 Vectors
6. GOLD Standard Protein Expression Products
7. Product Introductions
8. How can we help you?
inNovations 28, July 2008
1. Full issue, 1929 KB
2. NanoJuice™ Transfection Kits
3. Gateway� Nova DEST™ Vectors
4. KOD Xtreme™ Hot Start DNA Polymerase
5. ProteoExtract� Transmembrane Protein Extraction Kit
6. Benzonase� Nuclease
7. GeneJuice� Transfection Reagent
inNovations 27, March 2008
1. Full issue, 1857 KB
2. WideScreen Assays
3. New Size Available - D-Tube™ Dialyzer Mega Kits
4. InnoZyme™ Insulysin/IDE Immunocapture Activity Assay
5. BugBuster� Protein Extraction Reagent
6. Answers to questions about Proteases for Fusion Tag Removal
7. His•Tag� Antibody HRP Conjugate Kit & Microbiology Media: Terrific Broth and 2xYT
inNovations 26, September 2007
1. Full issue, 984 KB
2. NEW Overnight Express™ Autoinduction NMR Media
3. Pellet Paint� Co-Precipitant for microarray work
4. Product Introductions
5. Introducing GST•Bind™ Fractogel� Cartridges
6. Unique new kits for phosphopeptide enrichment
7. Product Updates
8. Citation Spotlight
inNovations 25, February 2007
1. Full issue, 2255 KB
2. iFOLD™ Protein Refolding System 2
3. Introducing the D-Tube96™ Dialyzer kits
4. KOD Hot Start - Fidelity with Speed
5. Introducing dual-purpose pIEx/Bac™ Vectors
6. InnoCyte™ Flow Cytometric Cytochrome c Release Kit
7. Complete K-LISA™ kit to measure mTOR kinase activity
8. ProteoPlex� Human Cytokine Array
9. Answers to questions about NoShift™ II Transcription Factor Assay Kits
inNovations 24, August 2006
1. Full issue, 1096 KB
2. NEW ProteoExtract� Kits
3. Faster, Easier - proteins from insect cells
4. iFOLD™ Protein Refolding System 1
5. Rosetta™ (DE3) Competent Cells
6. Transfection reagent comparison
7. NEW! NoShift™ CREB, FOXO-1a Reagents
8. Answers to questions about Selecting E. coli cloning hosts
inNovations 23, May 2006
1. Full issue, 1685 KB
2. iFOLD™ Protein Refolding System 1
3. Microarray for human angiogenic cytokines
4. Introducing MAC cartridges
5. NovaTaq™ Master Mixes
6. NEW Transcription factor assays
7. Enrich samples for phosphoproteins
8. Origami™ B(DE3) Competent Cells
9. Answers to questions about ProteoPlex� Arrays
inNovations 22, November 2005
1. Full issue, 1901 KB
2. Strep•Tag� II fusion tag provides rapid one-step affinity purification
3. New ProteoEnrich™ chromatography kits
4. Protein refolding database
5. New NoShift™ Transcription Factor Assay Reagents
6. Answers to questions about the Strep•Tag�/Strep•Tactin� affinity purification products
inNovations 21, July 2005
1. Full issue, 1323 KB
2. (451 KB): Autoinduce protein expression without IPTG Overnight Express™ Instant TB Medium
3. (222 KB): Low sample volume murine cytokine microarray ProteoPlex™ Microarray
4. (109 KB): Comparison of Insect Cell Expression Systems: Baculovirus and InsectDirect™
5. (71 KB): KOD Hot Start DNA Polymerase
6. (431 KB): New kits for dialysis and electroelution D-Tube™ Dialyzers and Electroelution Accessory Kit
7. (105 KB): Answers to questions about the Overnight Express Autoinduction Systems
inNovations 20, October 2004
1. Full issue, 1790 KB
2. (281 KB): New pET expression vectors and HRV 3C Protease for efficient fusion tag removal
3. (348 KB): Rapid, ELISA-based measurement of protein tyrosine kinase (PTK) activity
4. (409 KB): Phage library-based selection of immunogenic proteins in cancer patients
5. (213 KB): ProteoExtract^� Native Membrane Protein Extraction Kit
6. (480 KB): ProteoExtract^� Phosphopeptide Capture Kit
7. (101 KB): Preserve phosphorylation state and kinase activity in cell extracts
8. (62 KB): Selection Guide for His•Tag^� Fusion Protein Purification
inNovations 19, April 2004
1. Full issue, 2051 KB
2. (342 KB): A unique antibody microarray for multiplex analysis of human cytokines
3. (1022 KB): Affinity-based enrichment of ATP-binding proteins
4. (189 KB): The InsectDirect™ System: rapid, high-level expression and purification of proteins from insect cells
5. (130 KB): A convenient method and kit for DNA-directed siRNA expressionin vivo
6. (95 KB): A rapid procedure for protein extraction from filamentous fungi and plants using YeastBuster™ Protein Extraction Reagent
7. (153 KB): pCOLADuet™-1, a lower-copy, Kan^R vector option for coexpression in E. coli
8. (437 KB): New Products
inNovations 18, October 2003
1. Full issue, 1178 KB
2. (104 KB): Enhanced protein coexpression in E. coli
3. (178 KB): New coexpression vectors for expanded compatibilities in E. coli
4. (245 KB): A rapid method for simultaneously cloning two open reading frames for coexpression in E. coli
5. (203 KB): Identification of DNA binding proteins using the NoShift™ Transcription Factor Assay Kit
6. (135 KB): A novel buffer system for direct PCR from whole blood
7. (102 KB): Robotic solubility screening and purification of fusion proteins
8. (244 KB): New Products
inNovations 17, June 2003
1. Full issue, 1249 KB
2. (238 KB): Unattended high-density cell growth and induction of protein expression with the Overnight Express™ Autoinduction System
3. (206 KB): Fidelty and reliability in PCR using KOD Hot Start
4. (243 KB): Rapid microbial gene detection and amplification techniques using colony-direct PCR
5. (207 KB): Detection of Shiga toxin-producing E. coli using multiplex colony-direct PCR with KOD XL DNA Polymerase
6. (209 KB): Efficient production of recombinant plant chitinase with Novagen’s E. coli Origami™(DE3) Strain
7. (313 KB): Protein delivery into mammalian cells using ProteoJuice™ Protein Transfection Reagent
8. (404 KB): Improved immunohistochemical staining of fixed tissues
9. (244 KB): New Products
10. (470 KB): Product Spotlight: ProteoExtract™ Kits from Calbiochem
inNovations 16, March 2003
1. Full issue, 1013 KB
2. (249 KB): Greater yields of full-length proteins using the EcoPro™ T7 transcription/translation system
3. (321 KB): A novel high-level transient protein expression and purification system for Sf9 insect cells that facilitates automated screening
4. (273 KB): Automated solubility screening of recombinant proteins in a 96-well format
5. (359 KB): Simple,powerful promoter and enhancer analysis with the MightyLight™ System
6. (183 KB): MagPrep^� Bacterial Genomic DNA Kit: Efficient isolation of genomic DNA from Gram-positive and Gram-negative bacterial species
7. (312 KB): New Products
8. (53 KB): Application Spotlight: Extraction of proteins from three different yeast species using YeastBuster™ Protein Extraction Reagent
9. (141 KB): Application Spotlight: Use of BugBuster^� and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria
inNovations 15, November 2002
1. Full issue, 932 KB
2. (357 KB): Coexpression of multiple target proteins in E. coli
3. (570 KB): Use of T7 phage display to clone RNA-binding cDNAs
4. (242 KB): Rapid and convenient extraction of nuclear proteins using the NucBuster™ Protein Extraction Kit
5. (245 KB):Application Feature: Protein Extraction Reagents Application Guide
6. (304 KB): YeastBuster™ Protein Extraction Reagent for fast,efficient extraction of proteins from yeast
7. (297 KB): Centrifugation-free protein extraction and purification from total cultures of baculovirus-infected insect cells
8. (435 KB): New Products
inNovations 14, July 2002
1. Full issue, 840 KB
2. (75 KB): Automated purification of recombinant proteins in a 96-well format with RoboPop Kits and robotic sample processing
3. (64 KB): Novel single-copy pETcoco™ vector with dual controls for amplification and expression
4. (51 KB): Targeted suppression of gene expression using siRNA and RiboJuice™ siRNA Transfection Reagent
5. (47 KB): pET-44 series for Nus•Tag™-enhanced protein solubility and improved purification with His•Mag™ Agarose Beads
6. (62 KB): Protocols to detect Cre recombinase expression in transgenic mouse tissues by immunostaining and Western blotting
7. (78 KB): Product Spotlight: Cleavage of fusion proteins
8. (147 KB): New Products
inNovations 13, November 2001
1. Full issue, 408 KB
2. (193 KB): Extraction and purification of proteins from E. coli without harvesting cells
3. (53 KB): Domain mapping of nuclear transport factor
4. (51 KB): Expression and purification of insect cadherin ectodomain using the pTriEx™-2 multisystem vector in Origami™ and Tuner™ cells
5. (62 KB): Use of glucose to control basal expression in the pET System
6. (74 KB): Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips
inNovations 12, June 2001
1. Full issue, 565 KB
2. (125 KB): Overcoming the codon bias of E. coli for enhanced protein expression
3. (30 KB): Enhanced sensitivity of ChIP through the application of Pellet Paint^� Co-Precipitant
4. (36 KB): Simple,efficient extraction of protein from hepatocytes with CytoBuster™ Reagent
5. (59 KB): Sf9 insect cells and serum-free media adapted for different baculovirus applications
6. (71 KB): Western blotting and immunohistochemistry using the His•Tag^� Monoclonal Antibody
inNovations 11, June 2000
1. (140 KB): Mobius™ 1000 Plasmid Kits for convenient isolation of ultrapure plasmid DNA
2. (115 KB): Expression of soluble heterologous proteins via fusion with NusA protein
3. (102 KB): A superior E. coli coupled transcription-translation system for synthesis of full-length proteins from circular or linear DNA
4. (81 KB): New Products
inNovations 10, November 1999
1. (143 KB): pTriEx™-1 Multisystem Vector for protein expression in E. coli,mammalian,and insect cells
2. (102 KB): pETBlue™ Vectors: combining the utility of blue/white screening with the power of T7 promoter-based expression
3. (30 KB): FRET based homogenous assay of S•Tag™ fusion proteins
4. (43 KB): Efficient production of disulfide bonded proteins in the cytoplasm in ‘oxidizing’ mutants of E. coli
5. (24 KB):Technical Tip: Utilization of theNco I cloning site to express native target proteins >
6. (38 KB): Preparation of selenomethionine-labeled proteins using pET and E. coli
7. (59 KB): BugBuster™ and Benzonase^�: the clear solutions to simple,efficient extraction of E. coli proteins
8. (220 KB): Optimized automated sequencing with Pellet Paint^� NF Co-Precipitant
9. (133 KB): New Products
inNovations 9, January 1999
1. (135 KB): Non-chemical gene transfer into mammalian cells
2. (19 KB): Technical Tip: Use of "downstream" restriction enzyme cutters to generate any desired 4 bp 5′-overhang
3. (44 KB): Clonables™: a kit for fast,trouble-free ligation and transformation of any plasmid vector and insert
4. (13 KB): Technical Tip: Addition of glucose to growth media to reduce background expression in λDE3 lysogenic host strains
5. (59 KB): STP3™-Biotin: non-radioactive transcription/translation in a single tube
6. (52 KB): Pellet Paint^� Co-Precipitant minimizes false negatives in PCR assay
7. (66 KB): New Products
inNovations 8, September 1998
1. (160 KB): Simple,efficientin vitro protein expression with Single Tube Protein™ System 3
2. <(37 KB): Analysis of vitellogenin gene expression in individual pine weevils /a>
3. (42 KB): New pBAC transfer plasmids for production of secreted proteins
4. (59 KB): Ea4 insect cells for glycoprotein research
5. (79 KB): Efficient³²P-labeling of proteins expressed from pET-33b(+) for protein:protein interaction studies
6. (35 KB): Instant IMAC: pre-packed, pre-charged His•Bind Quick Cartridges and Columns
7. (78 KB): New Perfectly Blunt^� Kits and Quick Protocol: the fastest and most efficient PCR cloning system
8. (107 KB): New Products
inNovations 7, August 1997
1. (44 KB): Cellulose binding domains� A novel fusion technology for efficient,low cost purification and immobilization of recombinant proteins
2. (51 KB): New pET expression vectors generate fusion proteins with cellulose binding domains
3. (50 KB): New pBAC transfer plasmids for baculovirus expression of CBD fusion proteins
4. (89 KB): Purification and S•Tag detection of CBD fusion proteins
5. (69 KB): BacVector-3000: An engineered baculovirus designed for greater protein stability
6. (56 KB): New Products
inNovations 6, December 1996
1. (235 KB): T7Select Phage Display System: A Powerful New Protein Display System Based on Bacteriophage T7
2. (72 KB): Perfectly Blunt Cloning: A Superior Method for Cloning PCR Products or Any DNA
3. (27 KB): New Products
inNovations 5, August 1996
1. (114 KB): Immediate Early Baculovirus Vectors for Foreign Gene Expression in Transformed or Infected Insect Cells
2. (25 KB): Recombinant Enterokinase Cleavage of Fusion Proteins
3. (40 KB): Ligation Independent Cloning: Efficient Directional Cloning of PCR Products
4. (20 KB): Spectrophotometric Measurement of Nucleic Acids in the Presence of Pellet Paint™ Co-Precipitant
5. (26 KB): The Use of Pellet Paint^� as a Co-Precipitant in the Ribonuclease Protection Assay
6. (26 KB): Chemiluminescent Detection of S•Tag Fusion Proteins
7. (56 KB) New Products
inNovations 4a, May 1996
1. (152 KB): The BacVector System: Simplified Cloning and Protein Expression Using Novel Baculovirus Vectors
2. (48 KB): pET-33b(+): A pET Vector that Contains a Protein Kinase A Recognition Sequence
3. (46 KB): Lactose Fed-Batch Fermentation: A High-Yield Method Suitable for Use with the pET System
4. (62 KB): Pellet Paint^�: A Visible Nucleic Acid Carrier for Efficient,Error-Free Precipitation
5. (44 KB): New Products
inNovations 3, June 1995
1. (63 KB): A Novel Strategy for Directional Cloning of Random Primed cDNA
2. (24 KB): λSCREEN™: A High Efficiency cDNA Cloning Vector for Protein Expression,Purification and Subtractive Probe Analysis
3. (21 KB): Frequencies of Restriction Sites in Murine cDNA
4. (48 KB): pET TRX Fusion System for Increased Solubility of Proteins Expressed in E. coli
5. (18 KB): Frequently Asked Questions About cDNA Libraries
6. (414 KB): New Products
inNovations 2, November 1994
1. (127 KB): High-Yield Expression and Purification of Recombinant Peptides
2. (30 KB): Universal Western Blot Markers
3. (90 KB): Straight A�s™ mRNA Isolation System: Rapid,High-Quality Poly(A)+ RNA from Diverse Sources
4. (133 KB): New Products
inNovations 1, May 1994
1. (75 KB): The pET System: Your Choice for Expression
2. (136 KB): S•Tag™: A Multipurpose Fusion Peptide for Recombinant Proteins
3. (45 KB): pT7Blue T-Vector Stability
4. (59 KB): New Products