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InsectDirect™ System
 

Insect Cell Expression Vectors

The pIEx™ series of transient expression vectors are designed for rapid, high-level protein expression in insect cells, while eliminating the need to create recombinant baculovirus for protein expression. They feature an optimal combination of AcNPV baculovirus-derived transcription elements, the hr5 (homologous region 5) enhancer and the ie1 (immediate early) promoter, to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and its associated cytopathic effects.

The pBiEx™ series of multisystem expression vectors allows rapid characterization/expression of target genes in both E. coli and insect cells using the same construct. These vectors feature the same hr5 enhancer and the ie1 promoter as the pIEx vectors to direct expression in insect cells and also allow for expression in E. coli by the tightly controlled T7lac promoter.

 

Vector
Promoter(s)
Signal
sequence
Fusion Tags
Protease
cleavage
sites†
Ek/LIC
vector
option
N-terminal
C-terminal
pIEx-1
hr5/ie1
No
His•Tag®/S•Tag™HSV•Tag®
Tb/Ek
Yes††
pIEx-2
hr5/ie1
No
GST•Tag™/His•Tag/S•TagHSV•Tag
Tb/Ek
Yes††
pIEx-3
hr5/ie1
Yes
GST•Tag/His•Tag/S•TagHSV•Tag
Tb/Ek
Yes††
pIEx-4
hr5/ie1
No
NoneS•Tag/His•Tag
None
No
pIEx-5
hr5/ie1
Yes
None
S•Tag/His•Tag
None
No
pIEx-6
hr5/ie1
No
His•TagS•Tag
Ek
No
pIEx-7 Ek/LIC*
hr5/ie1
No
His•TagS•Tag
Ek
Yes
pBiEx-1
hr5/ie1, T7lac
No
His•Tag/S•TagHSV•Tag
Tb/Ek
No
pBiEx-2
hr5/ie1, T7lac
No
GST•Tag/His•Tag/S•TagHSV•Tag
Tb/Ek
No
pBiEx-3
hr5/ie1, T7lac
No
NoneS•Tag/His•Tag
None
No

* pIEx-7 is available only as an Ek/LIC vector. The pIEx-7 Ek/LIC Vector Kit includes Linearized Ek/LIC Vector; T4 DNA Polymerase, LIC-qualified; polymerase buffer; DTT, EDTA, and dATP solutions; Nuclease-free Water; competent cells; SOC Medium; and controls.
† Tb: thrombin; Ek: enterokonase
†† pIEx-1, pIEx-2, and pIEx-3 vectors are also available in an Ek/LIC Vector Kit. More information...