InsectDirect™ System  Insect Cell Expression Vectors The pIEx™ series of transient expression vectors are designed for rapid, high-level protein expression in insect cells, while eliminating the need to create recombinant baculovirus for protein expression. They feature an optimal combination of AcNPV baculovirus-derived transcription elements, the hr5 (homologous region 5) enhancer and the ie1 (immediate early) promoter, to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and its associated cytopathic effects.
The pBiEx™ series of multisystem expression vectors allows rapid characterization/expression of target genes in both E. coli and insect cells using the same construct. These vectors feature the same hr5 enhancer and the ie1 promoter as the pIEx vectors to direct expression in insect cells and also allow for expression in E. coli by the tightly controlled T7lac promoter. |