| pIEx/Bac Vectors | The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac vectors carries a Strep•Tag® II coding sequence (1) and a His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2).
1. Skerra, A., Schmidt, T.G.M. (2000) Meth. Enzymol. 326, 271–304.
2. Fiedler, M., Horn, C., Bandtlow, C., Schwab, M.E. (2002) Protein Eng. 15, 931–941. | | The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button. You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
| Vector | Sequence | Map | Ordering
Information | Newsletter
Articles | Technical
Bulletins | | pIEx/Bac-1 |  |  |  | | | | pIEx/Bac-1 Ek/LIC | | | | | | | pIEx/Bac-2 | | | | | | | pIEx/Bac-2 3C/LIC | | | | | | | pIEx/Bac-3 | | | | | | | pIEx/Bac-3 3C/LIC | | | | | | | pIEx/Bac-4 | | | | | | | pIEx/Bac-4 Ek/LIC | | | | | | | pIEx/Bac-5 | | | | | | | pIEx/Bac-5 3C/LIC | | | | | |
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