 |
|
 |
 |
 |
| pIEx and pBiEx Vectors | The pIEx™ vectors are designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. These vectors feature the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects.
The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button.You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
| Vector | Sequence | Map | Ordering Information | Newsletter Articles | Technical Bulletins | | pIEx-1 |  |  |  |  | | | pIEx-2 |  |  |  |  | | | pIEx-3 |  |  |  |  | | | pIEx-4 |  |  |  |  | | | pIEx-5 |  |  |  |  | | | pIEx-6 |  |  |  |  | | | pIEx-7 Ek/LIC |  |  |  |  | | | pIEx-8 |  |  |  | | | | pIEx-8 Ek/LIC |  |  |  | | | | pIEx-9 |  |  |  | | | | pIEx-9 3C/LIC |  |  |  | | | | pIEx-10 |  |  |  | | | | pIEx-10 Ek/LIC |  |  |  | | |
|
|