| Target genes are often expressed in more than one system for various purposes. For example, a bacterial system may be used for initial studies to ascertain solubility or activity, or to produce large amounts of protein for structural studies or antibody production. The same gene may be expressed in insect and/or mammalian cells, in order to obtain higher activity or eukaryotic post-translational modifications. With current expression vectors, it is necessary to clone the target gene into separate vectors, each dedicated to a particular system. Although Novagen's bacterial, baculovirus and mammalian expression vectors offer compatible cloning strategies and restriction sites to make the cloning process more convenient, the entire process can be streamlined by using a single vector to reliably express target genes in the three major expression systems. The benefits of such a multisystem vector are enhanced when performing high throughput gene analysis, which currently requires a significant effort to construct and manage all of the multiple recombinants used for different expression systems. To address this need, Novagen has developed the pTriEx™ System, a novel expression vector platform that enables optimal protein expression in bacterial, insect and mammalian cells from a single plasmid. The pTriEx system thus combines the proven features of the pET, BacVector™ and BacMam™ Systems and provides all of the benefits and versatility of each expression system in one vector backbone. The TriEx family of vectors has been extensively tested for performance with various target genes in all three expression systems. In each case, expression was similar to that obtained with system-specific vectors. The vector sequences are provided in GenBank format, with significant features indicated. To convert the sequence into other formats, first select the sequence and copy (Command-C on Mac or Ctrl-C on Windows). Next, click here to open the NIH ReadSeq web page in a new window. Paste the sequence into the input field (Command-V on Mac or Ctrl-V on PC), choose your favorite format from the dropdown menu, and hit the "submit" button. You can access sequences, maps, ordering information, newsletter articles and technical bulletins through the following links: |
