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Strep•Tactin
Strep•Tactin® Purification
  •  Strep•Tactin Matrix Selection Guide
  •  Purification Accessory Products
   Back to Strep•Tag/Strep•Tactin Affinity Purification page
 
Strep•Tactin resins offer rapid one-step affinity purification of proteins containing the Strep •Tag® II fusion tag while preserving the protein’s bioactivity. Two different types of resins of differing supports are available. The Strep•Tactin® Superflow™ Agarose is a cross-linked agarose derivatized with Strep•Tactin protein and can be used for gravity flow as well as for low pressure and FPLC chromatography. The Strep•Tactin® MacroPrep® Resin is a polymethacrylate resin suitable for gravity flow and all low pressure chromatography applications. This resin exhibits non-specific binding properties that differ from the Strep•Tactin Superflow™ Agarose and therefore is recommended when suboptimal results are obtained with the Superflow. These resins are also available in a variety of convenient formats, such as pre-packed columns, and cartridges, HT96™ plates and spin columns.
 
Strep•Tactin Matrix Selection Guide
 
ProductFormCapacityFeaturesApplication
Strep•Tactin SuperFlow™ Agarose* Superflow 6 Agarose50-100 nmol/ml Reusable 3-5 times; Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME Small to Production scale; FPLC or gravity flow column
Strep•Tactin SuperFlow Column*, 0.2 ml Superflow 6 Agarose, pre-packaged column 10-20 nmol Reusable 3-5 times; Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME Conevenient purification; gravity flow to column
Strep•Tactin SuperFlow Column*, 1 mlSuperflow 6 Agarose, pre-packaged column 50-100 nmolReusable 3-5 times; Luer fitting on one end; Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME Conevenient purification; gravity flow to column
Strep•Tactin SuperFlow Cartridge*, 1 mlSuperflow 6 Agarose, pre-packaged cartridge 50-100 nmolReusable 3-5 times; Compatible with Luer adaptors; Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME Syringe-driven processing; Vacuum manifold processing; Rapid purification
Strep•Tactin SuperFlow Cartridge*, 5 mlSuperflow 6 Agarose, pre-packaged cartridge 250-500 nmol Reusable 3-5 times; Compatible with Luer adaptors; Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME Syringe-driven processing; Vacuum manifold processing; Rapid purification
Strep•Tactin MacroPrep® Resin Polymethacrylate50-100 nmol/ ml Reusable 3-5 times; 50 um particle size; High flow rates and pressures; Compatible with 1M urea or guanidine, and 50 nM DTT or 2-ME Small to production scale; FPLC or gravity flow column
Strep•Tactin MacroPrep® Cartridge Polymethacrylate, pre-packaged cartridge 50-100 nmol Reusable 3-5 times; Compatible with Luer adaptors; 50 um particle size; High flow rates and pressures; Compatible with 1M urea or guanidine, and 50 nM DTT or 2-ME Syringe-driven processing; Vacuum manifold processing; FPLC/HPLC workstation; Rapid purification
Strep•Tactin HT96™ Purification Plate* Superflow 6 agarose 100 mg/well Compatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME High-throughout robotic processing; Vacuum manifold; Parallel processing
Strep•Tactin SpinPrep™ Column*
Coming Soon!
Agarose, pre-packed column 150 mgCompatible with 1M urea or guanidine, 0.5 M NaOH, 50% formamide, and 50 nM DTT or 2-ME High-throughout robotic processing; Vacuum manifold; Parallel processing
Note: The Strep•Tag/ Strep•Tactin purification system is designed for column purification and is not recommended to be used in batch purification.
Abbreviations: 2-ME: mercaptoethanol; DTT: Dithiothreitol
* manufactured by IBA
 
Purification Accessory Products
 
ProductSizeCat. No.
M6 Adaptor Set for FPLC 1 Kit 71586-3
¼-28 Adaptor Set for FPLC 1 Kit 71587-3
10-32 Adaptor Set for Äkta 1 Kit 71588-3
1/16 Inch Adaptor Set for peristaltic pump tubing 1 Kit 71589-3
Strep •Tactin Buffer Kit 1 Kit 71613-3
10X Strep •Tactin Wash Buffer 100 ml 71603-3
10X Strep •Tactin Elute Buffer 25 ml 71604-3
10X Strep •Tactin Regeneration Buffer 100 ml 71612-3
D-Desthiobiotin 1 g 71610-3