GeneJuice® Transfection Reagent DNA Transfection Reagent for mammalian cells
GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to lipid-based reagents, as well as traditional techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. Whereas many available transfection reagents are based on cationic lipid formulations, GeneJuice is composed of a nontoxic cellular protein and a small amount of a novel polyamine. The unique chemistry provides several advantages over lipid-based transfection, including: - Highly efficient DNA transfer for both stable and transient transfections
- Minimal cellular toxicity
- Compatibility with both serum-containing and serum-free media
- Simple protocol—no need for media changes
- Ideal for high throughput (HT) transfection in multi-well plate formats
GeneJuice Transfection Reagent provides excellent performance in both stable and transient transfection of mammalian cells and is ideal for use with reporter (pMLuc™), drug selection (pTK-neo), and expression (pTriEx™ and pTandem™-1), vectors. The 1-ml size provides enough reagent to perform up to 500 transfections in standard 35-mm plates. The reagent is also available in an introductory 0.3-ml size. GeneJuice is supplied as a ready-to-use sterile solution. See the citations listing the cell lines and primary cells successfully transfected with GeneJuice Transfection Reagent! |