Cat. No. HCS30 Format: 50 Tests
Positive Control: Any cell or tissue sample labeled with BrdU
Assay Time: ~2.5 hours plus labeling time
Sample Type: Frozen or paraffin-embedded tissue sections or fixed cell preparations
Comments: Many cell proliferation studies have used the incorporation of radioactive thymidine as evidence of DNA replication. As an alternative non-radioactive technique, bromodeoxyuridine (BrdU), a thymidine analog, can be incorporated into proliferating cells (S-phase) much like radioactive thymidine. The BrdU is then detected by a monoclonal anti-BrdU antibody and visualized using a highly sensitive streptavidin-biotin staining system. BrdU has proven useful for proliferative studies of normal and neoplastic tissues both in vivo and in vitro. The BrdU Immunohistochemistry System uses a biotinylated monoclonal anti-BrdU, eliminating the need for a species-specific antibody. As a result, BrdU labeling can be performed in rats and mice without problems related to cross-reactivity or non-specific background staining. Staining is visualized using streptavidin-peroxidase and diaminobendizine (DAB), which will stain BrdU-incorporated nuclei a dark brown. Easy to Use • Complete • Versatile • Sensitive |
