Product Spotlight: Just in time for taxes...IRS Antibodies!
Anti-Insulin Receptor Substrate-1, Rat (Mouse) Cat. No.: GR45 Host: Mouse Isotype: IgG Immunogen: A synthetic peptide (YYASINFQKQPEDRQ) corresponding to amino acid residues 1221 - 1235 of rat insulin receptor substrate-1 (IRS-1). Positive Control: HepG2 or 3T3-L1 cells or liver or muscle tissue. Negative Control: Normal Mouse Serum (Cat. No. NS03L) Comments: Recognizes a protein of 160-185 kDa depending on the cell type studied. IRS-1 is a major endogenous substrate for the insulin receptor and the IGF-IR tyrosine kinase.
Anti-Insulin Receptor Substrate, Phospho-Specific (Tyr891), Human (Rabbit) Cat. No.: PC463 Host: Rabbit Isotype: IgG Immunogen: A synthetic phosphopeptide [KKKSPGEpYVNIEFG(C)] corresponding to amino acid residues of human insulin receptor substrate-1 (hIRS-1). Positive Control: MCF7 or SW480 cells Negative Control: Normal Rabbit Serum (Cat. No. NS01L) Comments: Detection of phosphorylated hIRS is an indication of insulin receptor and insulin-like growth factor activity.
Anti-Insulin Receptor Substrate-1, Rat (Rabbit) Cat. No: 420292 Comments: Immunogen used was a GST-fusion protein containing amino acids 900-1235 of rat IRS-1. Detects a band at ~185 kDa corresponding to IRS-1. Upon insulin stimulation, IRS proteins are phosphorylated by insulin receptor tyrosine kinase. Decreased levels of IRS proteins are associated with insulin resistance in humans.
CHO/IR cells, unstimulated (-) and stimulated with insulin (+) were lysed and either immunoprecipitated with anti-IRS-1 or IRS-2 (as indicated) and then blotted with anti-IRS-1 (left four columns). Antibody is highly specific for IRS-1 and does not detect protein precipitated by IRS-2. Immunoprecipitation removes most all IRS-1 from the cell lysate (right 2 columns).
Anti-Insulin Receptor Substrate-2, Mouse (Rabbit) Cat. No.: 420293 Immunogen used was a GST-fusion protein containing amino acids 1122-1321 of mouse IRS-2. Detects a band at ~180 kDa corresponding to IRS-2. Upon insulin stimulation, IRS proteins are phosphorylated by insulin receptor tyrosine kinase. Decreased levels of IRS proteins are associated with insulin resistance in humans. IRS-2 can serve as an alternative insulin substrate in IRS-1 deficient mice.
CHO/IR cells, unstimulated (-) and stimulated with insulin (+) were lysed and either immunoprecipitated with anti-IRS-1 or IRS-2 (as indicated) and then blotted with anti-IRS-2 (left four columns). Antibody is highly specific for IRS-2 and does not detect protein precipitated by IRS-1. Immunoprecipitation removes most all IRS-2 from the cell lysate (right 2 columns).
Anti-Insulin Receptor Substrate-1, Rat (Mouse)
