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Apoptosis Resource: Measurement of Cell Proliferation
 | | | Measurement of Cell Proliferation
In multicellular organisms, a delicate balance between cell proliferation and cell death is maintained for normal cellular homeostasis, failure of this balance can lead to disease states. For example, on an average day humans produce ~60 x 109 new cells and at the same time eliminate roughly the same number of mature cells. Hence, a coupled relationship exists between proliferation and apoptosis. While certain morphological and biochemical changes serve as markers of apoptosis, cell proliferation can be used as another criteria for assessing apoptosis. Incorporation of the thymidine analog, bromodeoxyuridine (BrdU), into newly synthesized DNA strands of actively proliferating cells can be used as an alternative to radioisotopic methods for measuring cell proliferation. Following partial denaturation of double-stranded DNA, BrdU is detected immunochemically using an anti-BrdU antibody, allowing the assessment of the population of cells, which are actively synthesizing DNA. | | | | | | | Measurement of Cell Proliferation: Kits |
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Number of Tests or Samples |
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| BrdU Cell Proliferation Assay |
Colorimetric |
Intact Cells |
200 or 1000 Tests |
QIA58 |
| BrdU Cell Proliferation Assay, HTS |
Fluorometric |
Intact Cells |
200 or 1000 Tests |
HTS01 |
| BrdU Immunohistochemistry System |
Microscopy |
Paraffin-embedded tissues or fixed cells |
50 Tests |
HCS30 |
| Rapid Cell Proliferation Kit |
Colorimetric |
Cultured cells |
500 Tests |
QIA127 |
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| | | | | BrdU Cell Proliferation Assay
Cat. No. QIA58
BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.
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