Merck UK: Calbiochem - Phosphatase Inhibitor Cocktail Sets

		Technical Resources

		Technical Information

		Calbiochem Information

		Protease and Phosphatase Inhibitor Cocktails Resource

�

Protease Inhibitor Cocktails		Phosphatase Inhibitor Cocktails
•	Overview/Selection Guide	•	Overview/Selection Guide
•	Protease Inhibitor Cocktail Sets		Phosphatase Inhibitor Cocktail Sets
•	Properties of Selected Protease Inhibitors	•	Selected Protein Tyrosine Phosphatase Inhibitors
•	Frequently Asked Questions	•	Selected Serine/Threonine Phosphatase Inhibitors

�

Phosphatase Inhibitor Cocktail Set I (Cat. No. 524624)

A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml of phosphatase inhibitor cocktail reconstituted in DMSO. The concentrations of the individual inhibitors are shown in the table below. Note: 1 set = 5 x 1 ml. Not available for sale outside of the United States.

�

Phosphatase Inhibitor Cocktail Set II (Cat. No. 524625)

A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with tissue and cell extracts, including extracts containing detergents. Each vial contains 1 ml aqueous solution of the phosphatase inhibitor cocktail. The concentrations of the individual inhibitors are shown in the table below. Note: 1 set = 5 x 1 ml.

�

Phosphatase Inhibitor Cocktail Set III (Cat. No. 524627)

A cocktail of four phosphatase inhibitors for broadspectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials. Each vial contains 1 ml aqueous solution of the phosphatase inhibitor cocktail. The concentrations of the individual inhibitors are shown in the table below.

�

Phosphatase Inhibitor Cocktail Set IV (Cat. No. 524628)

A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials. Each vial contains a 1 ml solution phosphatase inhibitor cocktail. The concentrations of the individual inhibitors are shown in the table below.

�

Phosphatase Inhibitor Cocktail Set V, 50X (Cat. No. 524629) ^NEW!

A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials. Each vial contains 1 ml of aqueous solution with the following components: 250 mM Sodium Fluoride, 50 mM b-Glycerophosphate (Cat. No. 35675), 50 mM Sodium Pyrophosphate Decahydrate, 50 mM Sodium Orthovanadate. The concentrations of the individual inhibitors are shown in the table below.

�

PhosphoSafe™ Extraction Reagent (Cat. No. 71296)

PhosphoSafe™ Extraction Reagent efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formulation as CytoBuster™ Protein Extraction Reagent, but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, b-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications.

�