Merck UK: Calbiochem - Phosphatase Inhibitor Cocktails

		Technical Resources

		Technical Information

		Calbiochem Information

		Protease and Phosphatase Inhibitor Cocktails Resource

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Protease Inhibitor Cocktails		Phosphatase Inhibitor Cocktails
•	Overview/Selection Guide		Overview/Selection Guide
•	Protease Inhibitor Cocktail Sets	•	Phosphatase Inhibitor Cocktail Sets
•	Properties of Selected Protease Inhibitors	•	Selected Protein Tyrosine Phosphatase Inhibitors
•	Frequently Asked Questions	•	Selected Serine/Threonine Phosphatase Inhibitors

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Phosphatase Inhibitors Cocktails

Phosphorylation and dephosphorylation of proteins mediate signal transduction events that control a multitude of cellular processes such as cell division, cell proliferation, apoptosis, etc. Target proteins are phosphorylated by protein kinases that transfer a phosphate group to a specific protein, typically at serine, threonine, or tyrosine residues. The covalent attachment of a phosphoryl group changes the conformation of the protein and alters its ability to interact with a ligand. The phosphate group is removed by the action of protein phosphatases, that restore the protein to its original dephosphorylated state. During the extraction of phosphorylated proteins from cell and tissue lysates, it is highly critical that the phosphorylation state of the proteins be preserved. Calbiochem offers four different Phosphatase Inhibitor cocktails and a PhosphoSafe™ Extraction Reagent that help protect phosphoproteins from different families of phosphatases as shown in the selection guide below.

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Selection Guide for the Use of Specialized Phosphatase Inhibitor Cocktails

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