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Akt (pSer473) ELISA Data, Cat. No CBA005
Akt (pSer473) from extracts of Jurkat cells cultured in RPMI + 10% FCS were serially diluted in standard diluent buffer. The optical density of each dilution was plotted against the Akt (pSer473) standard curve. The data indicates that the standard accurately reflects full length Akt (pSer473) content from Jurkat samples.

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AKT ELISA Specificity
The specificity of this assay for Akt phosphorylated at serine 473 was confirmed by peptide competition. The data presented in figure 2 shows that only the phosphopeptide containing the phosphorylated serine could block the ELISA signal. The same sequence containing non-phosphorylated serine at position 473 did not block the signal. The assay was found to have no cross-reactivity with the following recombinant phosphoproteins tested at 100 ng/ml: p38 MAPK, p44 ERK1, p42 ERK2, JNK1, human insulin receptor, rat insulin receptor, and human HGFR (cmet).

 
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 Cat. No.
PhosphoDetect™ Akt (pSer473) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA005
PhosphoDetect™ Akt (pThr308) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA004
PhosphoDetect™ ATF2 (pThr69/71) ELISA Kit 96-well plate 96 Tests <1.0 U/ml 1.56-100 U/ml 4 h Cells CBA036
PhosphoDetect™ CREB (pSer133) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h CBA072
PhosphoDetect™ EGFR ELISA Kit 96-well plate 96 Tests 0.078 fmol/ml 0.078 - 5.0 fmol/ml 2.5 h Human serum or biological samples or cell lysates QIA95
PhosphoDetect™ ERK1/2 (pThr185/pTyr187) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA006
PhosphoDetect™ FAK (pTyr397) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h Cell lysate CBA062
PhosphoDetect™ GSK-3b (pSer9) ELISA Kit 96-well plate 96 Tests < 0.4 U/ml 1.6-100 U/ml 4 h CBA069
PhosphoDetect™ Hsp27 (pSer78/82) ELISA Kit 96-well plate 96 Tests 0.1-10 ng/ml 4.5 h Cell lysates CBA078
PhosphoDetect™ IkBa (pSer32) ELISA Kit 96-well plate 96 Tests < 1.0 Units/ml 1.6-100 Units/ml 4 h CBA065
PhosphoDetect™ Insulin Receptor (pTyr1162/1163) ELISA Kit 96-well plate 96 Tests <0.8 Units/ml 1.6-100 Units/ml 4 h Cells CBA038
PhosphoDetect™ JNK1/2 (pThr183/pTyr185) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA007
PhosphoDetect™ c-Kit (pTyr823) ELISA Kit 96-well plate 96 Tests <1.0 Unit/ml 1.6 - 100 Units/ml 4 h Cell lysate CBA075
PhosphoDetect™ MEK1 (pSer218/222) ELISA Kit 96-well plate 96 Tests ≤ 0.9 Units/ml 1.6-100 units/ml 3 h 20 min Cells CBA030
PhosphoDetect™ c-Met (pTyr1230/1234/1235) ELISA Kit 96-well plate 96 Tests <0.25 Units/ml 1.56-100 Units/ml 4 h Cells CBA073
PhosphoDetect™ p38 MAP Kinase (pThr180/pTyr182) ELISA Kit 96-well plate 96 Tests ≤0.8 U/ml 1.6-100 U/ml 4 h Cell lysates CBA008
PhosphoDetect™ p53 (pSer15) ELISA Kit 96-well plate 96 Tests ≤0.9 units/ml 1-100 units/ml 4 h Cell lysates CBA009
PhosphoDetect™ PRAS40 (pThr246) ELISA Kit 96-well plate 96 Tests < 0.9 units/ml 1.6-100 Units/ml 4 h Cell lysate, tissue extract CBA067
PhosphoDetect™ Src (pTyr 418) ELISA Kit 96-well plate 96 Tests ≤ 1 Unit/ml 1.6-100 Units/ml 4 h Cells CBA028
PhosphoDetect™ STAT1 (pTyr701) ELISA Kit 96-well plate 96 Tests < 0.9 Unit/ml 1.6-100 units/ml 4 h Cells CBA035
 
 
 
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