Merck UK: Calbiochem - Protease Inhibitor Cocktails

		Technical Resources

		Technical Information

		Calbiochem Information

		Protease and Phosphatase Inhibitor Cocktails Resource

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Protease Inhibitor Cocktails		Phosphatase Inhibitor Cocktails
•	Overview/Selection Guide	•	Overview/Selection Guide
	Protease Inhibitor Cocktail Sets	•	Phosphatase Inhibitor Cocktail Sets
•	Properties of Selected Protease Inhibitors	•	Selected Protein Tyrosine Phosphatase Inhibitors
•	Frequently Asked Questions	•	Selected Serine/Threonine Phosphatase Inhibitors

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Protease Inhibitor Cocktail Set I (Cat. No. 539131)

A cocktail containing five protease inhibitors that will inhibit a broad range of proteases. Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution. When diluted to 1X stock solution, each vial contains the concentrations of inhibitors shown in the table below.

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Protease Inhibitor Cocktail Set I, Animal-Free (Cat. No. 535142)

This animal-free formulation includes the same five protease inhibitors as the original version (Cat. No. 539131), but substitutes Aprotinin, Bovine Lung with a recombinant Aprotinin. Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution. Dilute to 1X before use.

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Protease Inhibitor Cocktail Set II (Cat. No. 539132)

This cocktail is recommended for use with bacterial cell extracts (except those intended for metal chelation chromatography). Cocktail contains five protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases. Reconstitute each vial with 1 ml DMSO and 4 ml H₂O to obtain a 5 ml stock solution. When reconstituted, each vial will contain the concentrations of inhibitors shown in the table below. One set is sufficient for about 20 g cells. Note: 1 set = 1 vial of lyophilized protease inhibitor cocktail and 1 vial DMSO, 1 ml.

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Protease Inhibitor Cocktail Set III, EDTA-Free (Cat. No. 539134)

This cocktail is recommended for use with mammalian cells and tissue extracts and for bacterial cell extracts being used for metal chelation chromatography. Cocktail contains six protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. Each vial contains the concentrations of inhibitors shown in the table below. One ml is sufficient for about 20 g tissue.

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Protease Inhibitor Cocktail Set III, Animal-Free (Cat. No. 535140)

This animal-free formulation includes the same six protease inhibitors as the original version (Cat. No. 539134), but substitutes Aprotinin, Bovine Lung with a recombinant Aprotinin. Each vial contains the concentrations of inhibitors shown in the table below. One ml is sufficient for about 20 g tissue.

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Protease Inhibitor Cocktail Set IV (Cat. No. 539136)

This cocktail is recommended for fungal and yeast cell extracts. Cocktail contains four protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of aspartic, cysteine, metallo-, and serine proteases. Each vial contains the concentrations of inhibitors shown in the table below.

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Protease Inhibitor Cocktail Set V, EDTA-Free (Cat. No. 539137)

An EDTA-free cocktail containing four protease inhibitors for the inhibition of serine and cysteine
proteases, but not metalloproteases. Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution. When diluted to 1X stock solution, the set will contain the concentrations of inhibitors shown in the table below.

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Protease Inhibitor Cocktail Set V, Animal-Free (Cat. No. 535141)

This animal-free formulation includes the same four protease inhibitors as the original version (Cat. No. 539137), but substitutes Aprotinin, Bovine Lung with a recombinant Aprotinin. Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution. Dilute to 1X before use.

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Protease Inhibitor Cocktail Set VI (Cat. No. 539133)

This cocktail is recommended for use with plant cell extracts. Cocktail contains six protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases. Each vial contains the concentrations of inhibitors shown in the table below. One ml is recommended for about 30 g of various plant tissues.

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Protease Inhibitor Cocktail Set VII (Cat. No. 539138)

This cocktail is recommended for purification of proteins containing His•Tag� sequences. Cocktail contains five protease inhibitors (in 1 ml DMSO) with broad specificity for the inhibition of cysteine, serine, aspartic, and thermolysin-like proteases and aminopeptidases. Each vial contains the concentrations of inhibitors shown in the table below. One ml is sufficient for about 10 g cells.

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Protease Inhibitor Cocktail Set VIII (Cat. No. 539129)

A DMSO solution of three protease inhibitors with selective specificity for the inhibition of cysteine proteases, including calpains, cathepsins, and papain. Each vial contains the concentrations of inhibitors shown in the table below.

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Serine Protease Inhibitor Cocktail Set I (Cat. No. 565000)

A cocktail of four protease inhibitors that is useful for the inhibition of a broad range of serine proteases. Reconstitute each vial with 1 ml H2O to obtain a 100X stock solution. When diluted to 1X stock solution, the set will contain the concentrations of inhibitors shown in the table below.

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Protease Inhibitor Set (Cat. No. 539128)

This unique set, consisting of seven different inhibitors, allows you to perform easy, economical screening for a suitable inhibitor for your application.

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Protease Arrest™ Reagent (Cat. No. 539124)

An optimized concentration of various reversible and irreversible inhibitors to inhibit serine, cysteine, and calpain proteases. Suitable for the protection of proteins purified from animal tissues, plant tissues, yeast, and bacteria. Protease Arrest Reagent is provided as a 50X solution that when diluted in extraction buffer at pH 7.0–8.0 inhibits 95–98% of protease activity. EDTA is also provided separately to inhibit metalloproteases.

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