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NEW! COX-2 ELISA Kit (Cat. No. CBA053)
 | | | Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins (PGs) from arachidonic acid. There are two isoforms of COX, COX-1 and COX-2. While COX-1 is expressed constitutively and appears to be responsible for the production of prostaglandins (PGs) that  control normal physiologic functions, expression of COX-2 is induced by various inflammatory and mitogenic stimuli such as cytokines, growth factors or tumor promoters. Numerous experimental studies suggest a relationship between COX-2 expression and carcinogenesis. For example, increased amounts of COX-2 have been observed in breast cancers that overexpress HER-2/neu. Furthermore, treatment with selective inhibitors of COX-2 reduced the formation of tongue, esophagal, intestinal, breast, skin, lung, and bladder tumors in experimental animals.
The human COX-2 ELISA is a complete kit for the quantitative determination of human COX-2 in cell lysates, tissue extracts, and biological fluids. The kit uses a rabbit anti-human COX-2 polyclonal antibody as the capture antibody. Test standards and samples are incubated in pre-coated well strips. Following a wash step, the detector antibody (monoclonal), followed by goat anti- mouse IgG conjugated to horseradish peroxidase (HRP), is added. The addition of the substrate and its subsequent reaction with the HRP creates a blue-colored solution. COX-2 levels are quantified by measuring absorbances at 450 nm (preferably with reference wavelength set at 540-600 nm) and comparing those values with the standard curve. | | | | The COX-2 ELISA Kit is: |  | Highly sensitive: detects COX-2 at 2.5–200 ng/ml | | Quantitative: includes recombinant COX-2 for standard curve comparisons | | Versatile: quantitates human COX-2 in cell lysates, tissue extracts, and biological fluids | | Convenient: 96-well, colorimetric detection |
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| | | | | | | | | Related Products | | Prostaglandin H Synthase-2, Human, Recombinant (Cat. No. 538950) | | Anti-COX2 Mouse mAb (5E10/D10) (Cat. No. 236004) | | Anti-COX2, C-Terminal Rabbit pAb (Cat. No. 236002) | | COX Inhibitors | | Related Products for Oxidative Stress Research | | | | |
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