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Protein A/G Affinity Resins
 | | | Protein A is a 42 kDa protein1,2 that is covalently linked to the peptidoglycan layer of the cell wall via the pentaglycin bridge.3 It contains little or no carbohydrate and has a protein structure that is readily accessible for cross-linking procedures. The protein can be obtained at very high purity by lysostaphin digestion of the bacteria followed by gel filtration and affinity chromatography on IgG-agarose. Protein A is stable over an extremely wide pH range (0.99 - 11.8) and is quite resistant to heat and other denaturing agents.4 Binding of protein A to the Fc region of the antibody is not affected by EDTA, non-ionic detergents, or temperatures up to 37°C. Furthermore, Protein A is easily renatured, even after treatment with 4 M urea, 6 M guanidine HCl, or 4 M thiocyanate.
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| Protein A Agarose |
Liquid slurry |
In 20% ethanol. |
AB PUR, IP |
539206 |
| Protein A Agarose |
Liquid slurry |
50% suspension in PBS. |
AB PUR |
IP06 |
| Protein A Agarose Suspension |
Liquid slurry |
In PBS. |
IP |
IP02 |
| Protein G-Agarose |
Liquid slurry |
50% suspension in PBS, 20% ethanol, pH 7.0. |
AB PUR, IP |
539207 |
| Protein G Plus-Agarose |
Liquid slurry |
50% suspension in PBS. |
AB PUR |
IP08 |
| Protein G Plus-Agarose Suspension |
Liquid slurry |
In PBS. |
IP |
IP04 |
| Protein G Plus/Protein A Agarose Suspension |
Liquid slurry |
33% slurry in PBS. |
IP |
IP05 |
| Protein G Plus/Protein A-Agarose |
Liquid slurry |
50% suspension in PBS. |
AB PUR |
IP10 |
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| | | | | | Related Resource | | Protein A, Protein G, and Related Products |
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