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Caspase Assays
 
Activation and Inhibition of Apoptosis
Apoptotic cell death has been characterized by a progressive series of morphological and biochemical changes ranging from the appearance on cell surfaces of phosphatidylserine, to proteolytic cleavage of numerous intracellular proteins, to nuclear condensation and fragmentation and the cleavage of DNA into nucleosomal fragments.1 Principally two mechanisms have been identified in mammalian cells for the induction of apoptosis: agents that lead to the perturbation of mitochondria, resulting in the leakage of cytochrome C and the activation of apoptosis; or agents that directly activate a family of death receptors leading to the activation of a parallel apoptotic cascade. Proapoptotic agents such as the activation of p53, growth factor withdrawal, irradiation, activated oxygen, and cytotoxic drugs induce their effects through the mitochondrial pathway.2 The mammalian proteins involved in this pathway have to a large extent been identified as homologs of the C. elegans genes originally identified as necessary for apoptotic control.3 Three proteins acted at the core of this pathway: Ced-3, Ced-4, and Ced-9. While Ced-3 was shown to be essential for the execution of apoptosis, the cytoplasmic protein Ced-4 was identified as an adapter protein that activated Ced-3.4 The mitochondrial protein Ced-9 in turn controlled the activation of Ced-4.5 Ced-3 produced the cellular cleavages necessary to induce apoptosis, and this protein was later shown to be a caspase.6
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Douglas K. Miller, Ph.D.
Department of Immunology and Rheumatology
Merck Research Laboratories
Rahway, NJ 07065
 
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Caspase Detection Kit (FITC-VAD-FMK) Flow Cytometry or Fluorimeter 100 Tests Fluorescence 1.5 h Intact cells QIA90
Caspase Detection Kit (Red-VAD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Intact cells QIA92
Caspase-8 Assay Kit 96-well plate 96 Tests Colorimetric or Fluorimetric 2.5-3.5 h Purified caspase-8 218770
Caspase-1 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests Colorimetric 2.5-3 h Cell lysates 218790
Caspase-1 Assay Kit, Fluorometric 96-well plate 100 Tests Fluorometric 3-3.5 h cell lysates 218791
Caspase-1 Inhibitor Screening Assay Kit 96-well plate 96 Tests Colorimetric 2.5-3 h Purified protein 218734
Caspase-2 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests Colorimetric 2.5-3 h Cell lysates 218792
Caspase-2 Assay Kit, Fluorometric 96-well plate 96 Tests Fluorometric 2.5-3 h Cell lysates 218793
Caspase-3 Activity Assay Kit, Fluorometric 96-well plate 96 Tests Fluorometric 2.5-3 h Cell lysates QIA70
Caspase-3 Activity Assay Kit, HTS 96-well plate 100 or 500 Tests Fluorometric 3 h Cultured cells or purified protein HTS02
Caspase-3 Cellular Activity Assay Kit 96-well plate 96 Tests Fluorometric or colorimetric 2.5-3 h Cell extracts 235419
Caspase-3 Detection Kit (FITC-DEVD-FMK) Flow cytometry, fluorescence microscopy 100 Tests Fluorescence 1.5 h Cultured cells QIA91
Caspase-3 Detection Kit (Red-DEVD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Cultured cells QIA93
Caspase-3 Immunoassay/Activity Kit, Fluorometric 96-well plate 96 Tests Fluorometric 6 h Cultured cells QIA107
Caspase-3 Inhibitor Screening Assay Kit 96-well plate 96 Tests Fluorometric or Colorimetric 2 h Recombinant or purified caspase-3 235418
Caspase-3 Intracellular Activity Assay Kit I (PhiPhiLux® G1D2) Flow cytometry or fluorescence microscopy 30 Tests Fluorescence 2 h Intact cells 235430
Caspase-3 Intracellular Activity Assay Kit II (PhiPhiLux® G2D2) Flow cytometry or fluorescence microscopy 30 Tests Fluorescence 1.5-2 h Intact cells 235432
Caspase-5 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests Colorimetric Cell lysate 218804
Caspase-6 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests Colorimetric 1.5-2.5 h Cell lysate 218802
Caspase-7 Immunoassay/Activity Kit, Fluorometric 96-well plate 96 Tests Fluorometric 6 h Cultured cells QIA108
Caspase-8 Activity Assay Kit, Fluorometric 96-well plate 96 Tests Fluorometric 2-3 h Cell lysate QIA71
Active Caspase-8 Assay Kit 96-well plate 182 Tests Fluorometric Cells CBA046
Caspase-8 Detection Kit (FITC-IETD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Intact cells QIA113
Caspase-8 Detection Kit (Red-IETD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Intact cells QIA114
Caspase-9 Activity Assay Kit, Fluorometric 96-well plate 96 Tests Fluorescence 2-3 h Cell lysate QIA72
Active Caspase-9 Assay Kit 96-well plate 480 Tests Fluorometric Cells CBA047
Caspase-9 Assay Kit, Colorimetric Cuvette or 96-well plate 100 Tests Colorimetric 2-3 h Cell lysate 218824
Caspase-9 Detection Kit (FITC-LEHD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Intact cells QIA115
Caspase-9 Detection Kit (Red-LEHD-FMK) Flow cytometry or fluorescence microscopy 100 Tests Fluorescence 1.5 h Intact cells QIA116
Caspase-10 Assay Kit, Fluorometric 96-well plate 96 Tests Fluorometric 2-3 h Cell lysates 218811
Active Caspase-3 ELISA Kit 96-well plate 96 Tests Colorimetric 3.5 h Cells CBA045
 
Related Resource
Apoptosis Pathway
 
 
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