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Protein Kinase Assays
 
Protein kinases are enzymes that phosphorylate target proteins, typically resulting in target activation. Because phosphorylation events regulate myriad signal transduction/disease pathways, kinases are attractive drug targets and the subject of intense research. Over the past 15 years, highly sensitive and specific methods have been created to monitor the level and activity of several kinases. As a result, a large selection of kinase assay and detection kits is available, offering increased flexibility for researching signaling pathways and kinase function. Due to the wide variety of kits, it is important to understand the format, strengths, limitations, and requirements of each assay.

Read more Kinase Kit Resource
 
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 Cat. No.
Akt Activity Immunoassay Kit Immunoblot 40 Tests 4 h Cell lysates, tissue extracts 124007
K-LISA™ Akt Activity Kit 96-well plate 96 Tests 0.05 mUnits 6-100 mU/well 3 h Cell lysates, tissue extracts, purified enzyme CBA019
PhosphoDetect™ Akt (pSer473) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA005
PhosphoDetect™ Akt (pThr308) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA004
TruLight™ Akt1/PKBa Kinase Assay Kit 96-well plate 96 Tests 25 pM 2 h Recombinant or purified enzyme 539705
PhosphoDetect™ ATF2 (pThr69/71) ELISA Kit 96-well plate 96 Tests <1.0 U/ml 1.56-100 U/ml 4 h Cells CBA036
TruLight™ Aurora A Kinase Assay Kit 96-well plate 96 Tests 6.9 pM 2 h recombinant or purified enzyme 539716
IP-Activity™ Bcr-Abl/c-Abl Kit CBA052
K-LISA™ Checkpoint Activity Kit 96-well plate 96 Tests 25 pg (0.018 mU) purified Chk1 25-725 µU (Chk1) and 260 µU - 26 mU (Chk2) 3 h Cell lysates, tissue extracts, purified enzymes CBA020
PhosphoDetect™ CREB (pSer133) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h CBA072
CREB ELISA Kit 96-well plate 96 Tests < 0.025 ng/ml 0.156-10 ng/ml 4 h Cells CBA071
PhosphoDetect™ EGFR ELISA Kit 96-well plate 96 Tests 0.078 fmol/ml 0.078 - 5.0 fmol/ml 2.5 h Human serum or biological samples or cell lysates QIA95
EGFR ELISA Kit 96-well plate 96 Tests 62.5-2000 pg/ml 6 h Cell or tissue extracts, serum, or plasma CBA018
c-ErbB2/c-Neu Rapid Format ELISA Kit 96-well plate 96 Tests 0-3 ng/ml 4 h Cell lysates, biological fluids QIA10
ERK 1/2 ELISA Kit 96-well plate 96 Tests < 16 pg/ml 31.2-2000 pg/ml 4 h Cells CBA032
TruLight™ ERK1/2 Assay Kit 96-well plate 96 Tests ERK1, 25 pM; ERK2, 66 pM 2 h recombinant or purified enzyme 539715
PhosphoDetect™ ERK1/2 (pThr185/pTyr187) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA006
FAK ELISA Kit 96-well plate 96 Tests < 1.6 ng/ml 1.6-100 ng/ml 4 h CBA063
PhosphoDetect™ FAK (pTyr397) ELISA Kit 96-well plate 96 Tests < 0.9 Units/ml 1.6-100 Units/ml 4 h Cell lysate CBA062
GSK-3b ELISA Kit 96-well plate 96 Tests < 0.26 ng/ml 0.39-25 ng/ml 4 h CBA068
PhosphoDetect™ GSK-3b (pSer9) ELISA Kit 96-well plate 96 Tests < 0.4 U/ml 1.6-100 U/ml 4 h CBA069
IkBa ELISA Kit 96-well plate 96 Tests < 30 pg/ml 62.5-4000 pg/ml 4 h CBA064
PhosphoDetect™ IkBa (pSer32) ELISA Kit 96-well plate 96 Tests < 1.0 Units/ml 1.6-100 Units/ml 4 h CBA065
K-LISA™ IKKb Inhibitor Screening Kit 96-well plate 96 Tests 5-10 ng Purified enzyme CBA044
Insulin Receptor (b-subunit) ELISA Kit 96-well plate 96 Tests < 0.5 ng/ml 0.94-60 ng/ml 4 h Cells CBA039
PhosphoDetect™ Insulin Receptor (pTyr1162/1163) ELISA Kit 96-well plate 96 Tests <0.8 Units/ml 1.6-100 Units/ml 4 h Cells CBA038
JNK Activity Immunoassay Kit Immunoblot 40 Tests Cell lysates 420115
JNK1/2 ELISA Kit 96-well plate 96 Tests 0.31-20 ng/ml 4 h Cells CBA033
PhosphoDetect™ JNK1/2 (pThr183/pTyr185) ELISA Kit 96-well plate 96 Tests ≤0.8 units/ml 1.6-100 units/ml 4 h Cell lysates CBA007
PhosphoDetect™ c-Kit (pTyr823) ELISA Kit 96-well plate 96 Tests <1.0 Unit/ml 1.6 - 100 Units/ml 4 h Cell lysate CBA075
c-Kit ELISA Kit 96-well plate 96 Tests < 15 pg/ml 19.5 - 1250 pg/ml 4 h Cell lysate CBA074
PhosphoDetect™ MEK1 (pSer218/222) ELISA Kit 96-well plate 96 Tests ≤ 0.9 Units/ml 1.6-100 units/ml 3 h 20 min Cells CBA030
MEK1 ELISA Kit 96-well plate 96 Tests < 0.1 ng/ml 0.16-10 ng/ml 4 h CBA070
c-Met ELISA Kit 96-well plate 96 Tests <4 ng/ml 0.78-50 ng/ml 4 h Cells CBA031
PhosphoDetect™ c-Met (pTyr1230/1234/1235) ELISA Kit 96-well plate 96 Tests <0.25 Units/ml 1.56-100 Units/ml 4 h Cells CBA073
K-LISA™ mTOR Activity Kit 96-well plate 96 Tests Purified or partially purified enzyme CBA055
TruLight™ p38a Kinase Assay Kit 96-well plate 96 Tests 211 pM 2 h recombinant or purified enzyme 539710
p38 MAP Kinase ELISA Kit 96-well plate 96 Tests ≤16 pg/ml 31.2-2000 pg/ml 4 h Cells CBA029
PhosphoDetect™ p38 MAP Kinase (pThr180/pTyr182) ELISA Kit 96-well plate 96 Tests ≤0.8 U/ml 1.6-100 U/ml 4 h Cell lysates CBA008
TruLight™ p70S6 Kinase Assay Kit 96-well plate 96 Tests 800 pM 2 h recombinant or purified enzyme 539711
TruLight™ Phosphoinositide 3-Kinase Assay Kit 384-well plate 384 Tests 2 h Purified enzyme 539718
PhosphoDetect™ Phosphoserine Detection Kit Lyophilized 525282
PhosphoDetect™ Phosphothreonine Detection Kit 525288
PhosphoDetect™ Phosphotyrosine Detection Kit 525322
Phosphotyrosine Molecular Weight Markers Lyophilized 525324
TruLight™ PKA Assay Kit 96-well plate 96 Tests 1.2 pM 2 h recombinant or purified enzyme 539712
TruLight™ PKCbI/II Assay Kit 96-well plate 96 Tests PKCβI, 4.2 pM; PKCβII, 2.2 pM 2 h recombinant or purified enzyme 539713
TruLight™ PKCa Assay Kit 96-well plate 96 Tests 2.5 pM 2 h recombinant or purified enzyme 539707
TruLight™ PKCe Assay Kit 96-well plate 96 Tests 7.6 pM 2 h recombinant or purified enzyme 539708
PhosphoDetect™ PRAS40 (pThr246) ELISA Kit 96-well plate 96 Tests < 0.9 units/ml 1.6-100 Units/ml 4 h Cell lysate, tissue extract CBA067
PRAS40 ELISA Kit 96-well plate 96 Tests <200 pg/ml 0.313-20 ng/ml 4 h CBA066
Protein Kinase Assay Kit, Non-Radioactive 96-well plate 96 Tests Cell lysates, purified enzymes 539484
TruLight™ Protein Substrate Kinase Assay Kit 96-well plate 96 Tests 2 h Purified enzyme 539719
Protein Tyrosine Kinase Positive Control, Rat Spleen Extract 325540
K-LISA™ PTK E4Y Reaction Plate 96-well plate 539702
K-LISA™ PTK EAY Reaction Plate 96-well plate 539703
K-LISA™ PTK EY Reaction Plate 96-well plate 539704
K-LISA™ PTK Screening Kit 96-well plate 216 Tests, 72 Controls 3 h Cell lysates, tissue extracts, purified enzyme 539701
TruLight™ RSK-2 Assay Kit 96-well plate 96 Tests 75 pM 2 h recombinant or purified enzyme 539709
Src ELISA Kit 96-well plate 96 Tests ≤1 ng/ml 1.6 - 50 ng/ml 4 h Cell lysates CBA027
TruLight™ Src Kinase Assay Kit 96-well plate 96 Tests 31.6 pM 2 h recombinant or purified enzyme 539706
IP-Activity™ Src Kit Cell lysates CBA056
PhosphoDetect™ Src (pTyr 418) ELISA Kit 96-well plate 96 Tests ≤ 1 Unit/ml 1.6-100 Units/ml 4 h Cells CBA028
PhosphoDetect™ STAT1 (pTyr701) ELISA Kit 96-well plate 96 Tests < 0.9 Unit/ml 1.6-100 units/ml 4 h Cells CBA035
STAT1 ELISA Kit 96-well plate 96 Tests <0.27 ng/ml 0.3-20 ng/ml 4 h Cells CBA034
TruLight™ Universal Kinase/Phosphatase Assay Kit 96- or 384-well plate 400 tests 2 h recombinant or purified enzyme 539714
 
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