Merck UK - Albumin/IgG Removal Kits

		Technical Resources

		Technical Information

		Calbiochem Information

		Kit Resource

		Protein Extraction & Sample Preparation

Albumin/IgG Removal Kits

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A major challenge for disease marker identification in human serum or plasma is the extraordinary range of protein abundance present in the sample. Proteins in plasma differ in concentration by a factor of one billion. Serum albumin can constitute 55% of total serum proteins and IgG can be as much as 25%. The presence of these proteins makes the resolution of lower-abundance proteins difficult. The new ProteoExtract^� Abundant Protein Removal Kits provide highly specific and efficient depletion of either albumin alone or albumin and IgG from serum, plasma, or cerebrospinal fluid (CSF). Removal of serum albumin and IgG eliminates approximately 75% of the total protein present in serum. A sample enriched three to four times can be loaded on 2D gels or liquid chromatography columns. Sample complexity is significantly reduced, thereby improving the detection and analysis of low-abundance proteins and peptides in biomarker discovery, toxicological studies for new drugs, protein profiling using SELDI analysis, and other applications.

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