Merck UK - Protein Extraction & Fractionation

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		Technical Information

		Calbiochem Information

		Kit Resource

		Protein Extraction & Sample Preparation

Protein Extraction & Fractionation

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ProteoExtract Kits are unique tools for differential display proteomics studies that allow analysis of complete or partial proteomes of bacterial, yeast, or mammalian cells, and of mammalian cells in normal versus diseased or disease-induced states. The Complete Proteome Extraction Kits (C-PEK) are designed for total protein extraction from bacterial, yeast, and mammalian cells. The Partial Proteome Extraction Kits (P-PEK) are designed for sequential extraction of protein fractions from bacterial, yeast, and mammalian cells according to differences in protein solubility. Both types of kits produce proteome samples ready for 2D gel electrophoresis and mass spectrometry. The Subcellular Proteome Extraction Kit (S-PEK) is designed for sequential extraction of protein fractions from mammalian cells according to their subcellular localization. The S-PEK produces native, functional proteins that can be used in activity assays, microarrays, and 1D and 2D gel electrophoresis. ProteoExtract Native Membrane Protein Extraction Kit (M-PEK) is designed for the isolation of non-denatured integral membrane or membrane-associated proteins from mammalian cells and tissues.

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