Myeloperoxidase [MPO, (EC1.11.1.7)] is one of a number of enzymes located in the azurophil granules of polymorphonuclear leukocytes (PMNs, also called neutrophils) and, to a lesser extent, in monocytes. MPO catalyzes the formation of hypochlorous acid (HOCl), a powerful oxidant formed from chloride ions and hydrogen peroxide. MPO generates reactive oxygen species as part of its function in innate host defense mechanisms, however, in an unregulated state, it is capable of causing tissue destruction, inflammation, and has been implicated in numerous diseases, including atherosclerosis, cancer, and Alzheimer’s disease.
The InnoZyme™ Myeloperoxidase Activity Kit is a sensitive and specific kit for quantitative measurements of human MPO activity in a variety of cell lysates and fluids. The kit may also be used to screen enzyme inhibitors. The InnoZyme Myeloperoxidase assay uses a specific polyclonal antibody immobilized on a 96-well plate to capture MPO. Activity is detected by colorimetric reaction.
- Highly selective: based on immunocapture of human MPO
- Highly sensitive and quantitative: assay range 5–100 ng/ml
- Versatile: measures human MPO activity in a variety of cell lysates and biological samples
- Convenient: 96-well format and non-radioactive detection
- Useful: enables high-throughput screening of human MPO inhibitors
