Antibody Guarantee

Antibody Guarantee - ELISA

Please complete the following questionnaire in as much detail as possible, (fields marked * are required) and click "submit" to send it to our technical team.

1. What are the catalogue number and the lot number of the product giving the problem?

Catalogue Number*
Lot Number*

�

2. At what temperature is the antibody being stored?*

�

3. Please describe the problem:*

�

4. What type of ELISA are you performing?* (eg Sandwich ELISA)

�

5. What type of cell line or tissue were the samples and what species were they?

Cell line or tissue
Species*

�

6. Please describe the coating conditions? * (buffer used, concentrations etc)

�

7. Please describe the blocking conditions* (which blocking agent, concentration, incubation time etc)

�

8. What concentration(s) of primary antibody have you tried?*

�

9. What concentration(s) of secondary antibody have you tried?*

�

10. Please describe the detection method that was used: *

�

11. What was your positive control?*

�

12. What was your negative control?*

�

13. Did the lot ever work?
That is did it work originally and suddenly ‘go bad’ or did it not work right from the start?

�

14. Have you used this product in the past in this procedure?

�

15. When was the antibody purchased?

�

16. Any other information:

�

Please fill out your contact details below:

Your Name:*
Department:*
Institution:*
Postcode:*
Telephone Number:*
Email: *

We will not use your email address for any purpose other than to contact you about this matter.

By completing this form you are authorizing Merck Biosciences and its affiliates to share this information solely with authorized distribution partners of Chemicals Ltd.. This information will not be shared with any other third party according to our privacy policy.

�