Antibody Guarantee - Western Blotting Contact Form

Antibody Guarantee - Western Blotting

Please complete the following questionnaire in as much detail as possible, (fields marked * are required) and click "submit" to send it to our technical team.

1 . What are the catalogue number and the lot number of the antibody?

Catalogue Number*
Lot Number*

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2. Did the proteins transfer from the gel to the membrane?
(prestained markers help, as does RedAlert stain)

	Yes
	No
	Not tested

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3. What concentration of primary antibody was used?*

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4. How long was the primary antibody incubation time?*

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5. What type of detection system was used?

	Colorimetric
	Chemiluminescent

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6. Was the Calbiochem Western Blotting Procedure followed?

	Yes
	No, please describe protocol:

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7. How long did it take for the blot to develop?*

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8. Approximately how many cells were used to make the lysate?

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9. How many �ls of lysate were loaded into each gel lane (or how much protein was loaded per lane)?

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10. What type of sample was tested?

Species*
Cell line/tissue

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11. What positive control was done and what were the results?*

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12. What negative control was done and what were the results?*

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13. Were the secondary reagents tested with any other primary antibodies?

Yes

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14. Please describe the problem:*

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15. At what temperature is the product being stored?*

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16. Did the lot ever work?
That is did it work originally and suddenly ‘go bad’ or did it not work right from the start?

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17. Have you used this product in the past in this procedure?

Yes

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18. When was the antibody purchased?*

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19. Any other information:

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Please fill in your contact details below:

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Your Name:*
Department:*
Institution:*
Postcode:*
Telephone Number:*
Email: *
We will not use your email address for any purpose other than to contact you about this matter.

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By completing this form you are authorizing Merck Chemicals Ltd. and its affiliates to share this information solely with authorized distribution partners of Merck Biosciences. This information will not be shared with any other third party according to our privacy policy.

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