Protein Refolding Temperature Following addition of the target protein, the refolding plate is incubated at 4-22°C for approximately 20 h. iFOLD® System 2 buffers maintain a relatively constant pH between 4 and 22°C, facilitating refolding experiments at different temperatures to accommodate the requirements of different target proteins. For many proteins, refolding at 22°C results in a greater fraction of active protein than refolding at lower temperatures. If the target protein isthermally unstable or particularly difficult to refold, refolding can be done at lower temperatures (4-10°C)
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