AP1062
PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb
Anti-Pyruvate Dehydogenase pSer293 Rabbit pAb
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10 September 2010
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Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse, primate, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HEK293 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue or Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human PDH-E1α, phosphorylated at Ser<sup>293</sup>, by immunoblotting. Samples: Whole cell lysate (20 µg) from HEK293 cells incubated in complete growth medium (lane 1) and incubated in complete growth medium and treated with dichloroacetate (DCA, 5 mM for 4 h). Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) (0.25 µg/ml). Detection: chemiluminescence. Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego.  Detection of PDH-E1α, phosphorylated at Ser<sup>293</sup> by immunocytochemistry. Samples: COS7 cells incubated in the absence (top 3 panels) and presence (middle 3 and bottom 3 panels) dichloroacetate (DCA; 5 mM for 2 h). All samples were incubated with MitoTracker<sup>®</sup> Red stain (100 nM for 20 min; to visualize mitochondria) and fixed in 3.7% formaldehyde, followed by methanol (3 min at -20°C), and permeabilization in PBS, 6% BSA, 0.3% Triton™ X-100 detergent, 0.1% Tween<sup>®</sup> 20 detergent. Primary antibodies: PhosphoDetect™ Anti-PDH-E1α (pSer<sup>293</sup>) Rabbit pAb (Cat. No. AP1062) (0.5 µg/ml, green fluorescence, top 3 and middle 3 panels) or anti-PDH-E1α antibody (green fluorescence, bottom 3 panels). Detection: fluorescence (Alexa Fluor<sup>®</sup> 488 secondary antibody) with DAPI counterstain (300 nM, 1 min, blue fluorescence). Data courtesy of Sandra Wiley and Matthew Rardin, University of California San Diego. |
