F-12509A Cyclic Product K1 Yellow powder. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A cell-permeable tetracyclic quinone compound that acts as a specific, reversible, and non-competitive inhibitor of CerK (ceramide kinase) activity (IC50 ~5 µM) with little effect against SPHK1/2 or DGKg (IC50 >100 µM). Shown to reduce cellular C1P (ceramide-1-phosphate) synthesis by 40% at ≥20 µM in a rat basophilic leukemia cell line RBL-2H3 and block CerK-mediated degranulation in both RBL-2H3 and murine BMMC (bone marrow-derived mast cells) in a dose-dependent manner. Exhibits no cytotoxic effect against RBL-2H3 even at concentrations as high as 100 µM. Purity: ≥95% by NMR (mixture of enantiomers). Ref.: Kim, J.W., et al. 2007. Biochim. Biophys. Acta 1771, 1262. Kumada, H., et al. 2007. Biosci. Biotechnol. Biochem. 71, 2581 Kim, J.W., et al. 2005. Biochim. Biophys. Acta 1738, 82.
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