AIM-1
Aurora-related Kinase 2
STK-1

Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween^®-20 detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human Aurora B kinase consisting of mino acids 2-344 fused at the N-terminus to a GST-His•Tag^® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Aurora B is a mitotic kinase that phosphorylates histone H3 and CenpA. Upregulation of Aurora B is reported in pancreatic and prostate cancers. The active kinase is useful in the study of Aurora B regulation and for inhibitor screening. Specific activity: ≥12 units/µg protein. One unit is defined as the amount of enzyme that is required to phosphorylate 1 pmol Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4. Purity: ≥70% by SDS-PAGE.

Ref.: Delaval, B., et al. 2004. Oncogene 23, 4516. Yasui, Y., et al. 2004. J. Biol, Chem. 279, 12997.
S: 26-45