Glycogen Synthase Kinase 3a Liquid. In 100 mM NaCl, 25 mM Tris-HCl, 10 mM reduced glutathione, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human GSK3a fused at the N-terninus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. GSK3a is unique in its preference for a priming phosphate at n+4 (where n is the site of phosphorylation by GSK3a) in order to phosphorylate many of its substrates. This motif is found in several well-established substrates of GSK3a, such as glycogen synthase, the epsilon subunit of eIF2B, and ATP citrate-lyase. The active kinase is useful in the study of GSK3a regulation and for inhibitor screening. Specific activity: ≥700 units/mg protein. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol glycogen synthase peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE) per min at 30°C, pH 7.4. Purity: ≥80% by SDS-PAGE. Ref.: Meijer, L., et al. 2004. Trends Pharmacol Sci. 25, 471. Patel, S., et al. 2004. Biochem Soc. Trans. 32, 803.
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