HDAC6, Human, Recombinant

Liquid. In 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween^®-20 detergent, 10% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening. Specific activity: ≥50 units/µg protein. One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C. Purity: ≥80% by SDS-PAGE.

Ref.: Pandey, U.B., et al. 2007. Nature 447, 859. Hubbert, C., et al. 2002. Nature 417, 455.