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TB047 pET-17b Vector Map
TB053 Academic and Non-Profit Laboratory Assurance Letter (Brookhaven Agreement)
TB055 pET System Manual, 11th Ed. PDF
pET-17b Sequence
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  pET-17b DNA
Cat. No. 69663  
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Prokaryotic Expression » pET Vector DNA
pET Vector DNA
Individual plasmid DNAs
 
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available on the Novagen website: www.novagen.com/vector 
 
Product Description

The pET-17b vector carries an N-terminal 11aa T7•Tag® sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker (1). Unique sites (except for the two BstX I sites) are shown on the vector map (TB047). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.

References:
1. Seed, B. (1987) Nature 329, 840.  

Need additional information about this product? Email our Technical Service department at: techservice@merckbio.eu

 Related information for this product is available:

Merck Chemicals Ltd. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Cat. No.
Size
In Stock
Qty
Select Country Above
For Pricing Information
69663-310 µgY$159.00*

* Additional Product Information
Cat. No.Disclaimers
69663-3
Brookhaven National LabsThis product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

Merck Chemicals Ltd. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Product Name
Cat. No.
Size
In Stock
Qty
Select Country Above
For Pricing Information
pET-3a DNA69418-310 µgY$122.00*
pET-3b DNA69419-310 µgY$122.00*
pET-3c DNA69420-310 µgY$122.00*
pET-3d DNA69421-310 µgY$122.00*
pET-9a DNA69431-310 µgY$122.00*
pET-9b DNA69432-310 µg*$122.00*
pET-9c DNA69433-310 µgY$122.00*
pET-9d DNA69434-310 µgY$122.00*
pET-11a DNA69436-310 µgY$122.00*
pET-11b DNA69437-310 µgY$122.00*
pET-11c DNA69438-310 µgY$122.00*
pET-11d DNA69439-310 µgY$122.00*
pLysE DNA69658-31 µgY$76.00*
pLysS DNA69659-31 µgY$76.00*
pET-14b DNA69660-310 µgY$159.00*
pET-15b DNA69661-310 µgY$159.00*
pET-16b DNA69662-310 µgY$159.00*
pET-19b DNA69677-310 µgY$159.00*
pET-20b(+) DNA69739-310 µgY$159.00*
pET-21a(+) DNA69740-310 µgY$159.00*
pET-21b(+) DNA69741-310 µgY$159.00*
pET-21c(+) DNA69742-310 µgY$159.00*
pET-21d(+) DNA69743-310 µgY$159.00*
pET-22b(+) DNA69744-310 µgY$159.00*
pET-23a(+) DNA69745-310 µgY$159.00*
pET-23b(+) DNA69746-310 µgY$159.00*
pET-23c(+) DNA69747-310 µg*$159.00*
pET-23d(+) DNA69748-310 µg*$159.00*
pET-24a(+) DNA69749-310 µgY$159.00*
pET-24b(+) DNA69750-310 µgY$159.00*
pET-24c(+) DNA69751-310 µgY$159.00*
pET-24d(+) DNA69752-310 µgY$159.00*
pET-25b(+) DNA69753-310 µgY$159.00*
pET-21(+) DNA69770-310 µgY$159.00*
pET-23(+) DNA69771-310 µgY$159.00*
pET-24(+) DNA69772-310 µgY$159.00*
pET-26b(+) DNA69862-310 µgY$159.00*
pET-27b(+) DNA69863-310 µgY$159.00*
pET-28a(+) DNA69864-310 µgY$159.00*
pET-28b(+) DNA69865-310 µgY$159.00*
pET-28c(+) DNA69866-310 µgY$159.00*
pET-29a(+) DNA69871-310 µgY$159.00*
pET-29b(+) DNA69872-310 µgY$159.00*
pET-29c(+) DNA69873-310 µgY$159.00*
pET-30a(+) DNA69909-310 µgY$159.00*
pET-30b(+) DNA69910-310 µgY$159.00*
pET-30c(+) DNA69911-310 µgY$159.00*
pET-31b(+) DNA69952-310 µgY$178.00*
pLacI DNA70610-31 µgY$76.00

* Additional Product Information
Cat. No.Disclaimers
69432-3
69747-3
69748-3
Stock StatusPlease contact our Customer Service Department for delivery time.
69418-3
69419-3
69420-3
69421-3
69431-3
69432-3
69433-3
69434-3
69436-3
69437-3
69438-3
69439-3
69658-3
69659-3
69660-3
69661-3
69662-3
69677-3
69739-3
69740-3
69741-3
69742-3
69743-3
69744-3
69745-3
69746-3
69747-3
69748-3
69749-3
69750-3
69751-3
69752-3
69753-3
69770-3
69771-3
69772-3
69862-3
69863-3
69864-3
69865-3
69866-3
69871-3
69872-3
69873-3
69909-3
69910-3
69911-3
69952-3
Brookhaven National LabsThis product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

Material Safety Data Sheets:
69663: pET-17b DNA - English
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Related Categories:
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Prokaryotic Expression » pET Vector DNA
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Prokaryotic Expression » pET Expression Systems 3, 9, 11, and 17b
All Categories » Novagen » Vectors » Plasmid » pET

Selected Citations:
  1. Heidi A. Erickson, Michelle D. Jund and Christopher A. Pennell. (2006) Cytotoxicity of human RNase-based immunotoxins requires cytosolic access and resistance to ribonuclease inhibition. Protein Engineering Design and Selection 19, 37-45.
  2. Karl J. Aichberger, et al. (2005) Hom s 4, an IgE-reactive autoantigen belonging to a new subfamily of calcium-binding proteins, can induce Th cell type 1-mediated autoreactivity. Journal of Immunology 175, 1286-1294.
  3. Yong Kim, et al. (2005) Mutation of cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect. Journal of Biological Chemistry 280, 23273-23279.
  4. Georg W. Mayr, Sabine Windhorst and Kirsten Hillemeier. (2005) Antiproliferative plant and synthetic polyphenolics are specific inhibitors of vertebrate inositol 1,4,5-trisphosphate 3-kinases and inositol polyphosphate multikinase. Journal of Biological Chemistry 280, 13229-13240.
  5. Shigetarou Mori, et al. (2005) Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitution. Journal of Biological Chemistry 280, 24104-24112.
  6. Motoshi Suzuki, et al. (2005) Novel structure of the N-terminus in yeast FIS1 correlates with a specialized function in mitochondrial fission. Journal of Biological Chemistry 280, 21444-21452.
  7. Bin Zhao, et al. (2005) Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. Journal of Biological Chemistry 280, 11599-11607.
  8. James S. Parker, S. Mark Roe and David Barford. (2004) Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. European Molecular Biology Organization Journal 23, 4727-4737.
  9. M. Schuster, M. L. Urbanowski and E. P. Greenberg. (2004) Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Procedings of the National Academy of Science 101, 15833-15839.
  10. Barsanjit Mazumder, et al. (2003) Regulated release of L13a from the 60S ribosomal subunit as a mechanism of transcript-specific translational control. Cell 115, 187-198.

...see all selected citations...