| | His•Tag® Monoclonal Antibody |
|  | Cat. No. 70796 |
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Sensitive, specific detection of His•Tag fusion proteins The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution. * Not recommended for HRP-based detection of Trail Mix Western Markers.
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Merck Chemicals Ltd. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
Merck Chemicals Ltd. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| His•Tag® AP Western Reagents | 70972-3 | 25 blots | Y | N/A | | His•Tag® AP LumiBlot Reagents | 70973-3 | 25 blots | * | N/A | | His•Tag® HRP LumiBlot Reagents | 70974-3 | 25 blots | Y | N/A |
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* Additional Product Information
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| - Alexander Schwendemann, et al. (2008) Hip, an HP1-interacting protein, is a haplo- and triplo-suppressor of position effect variegation. Procedings of the National Academy of Science 105, 204-209.
- Antonella Antignani and Richard J. Youle. (2007) The cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), can deliver Bcl-XL as an extracellular fusion protein to protect cells from apoptosis and retain differentiation induction. Journal of Biological Chemistry 282, 11246-11254.
- Valentina A. Shchedrina, et al. (2007) Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif. Procedings of the National Academy of Science 104, 13919-13924.
- Claire Vincent, et al. (2007) Activation of p61Hck triggers WASp- and Arp2/3-dependent actin-comet tail biogenesis and accelerates lysosomes. Journal of Biological Chemistry 282, 19565-19574.
- Wilfried Weber, et al. (2007) A synthetic time-delay circuit in mammalian cells and mice. Procedings of the National Academy of Science 104, 2643-2648.
- Martin Gengenbacher, et al. (2006) Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. Journal of Biological Chemistry 281, 3633-3641.
- Michael Krauss, et al. (2006) Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2µ-cargo complexes. Procedings of the National Academy of Science 103, 11934-11939.
- Luis Ojeda, et al. (2006) Role of glutaredoxin-3 and glutaredoxin-4 in the iron regulation of the Aft1 transcriptional activator in Saccharomyces cerevisiae. Journal of Biological Chemistry 281, 17661-17669.
- Brian Adams, et al. (2005) A novel class of dual-family immunophilins. Journal of Biological Chemistry 280, 24308-24314.
- Karl J. Aichberger, et al. (2005) Hom s 4, an IgE-reactive autoantigen belonging to a new subfamily of calcium-binding proteins, can induce Th cell type 1-mediated autoreactivity. Journal of Immunology 175, 1286-1294.
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