The pIEx/BacTM-5 vector contains an N-terminal GST•Tag™ coding sequence followed by a recognition site for HVR 3C protease. The multiple cloning region is followed by an optional C-terminal Strep•Tag® II coding sequence. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus.