The Gateway® Nova pET-58-DEST™ vector is designed to create expression clones (pEXPR) that are fused to N-terminal His•Tag® and Nus•Tag™ coding sequences and a thrombin cleavage site. The Nus•Tag fusion is included to enhance the solubility of the fusion protein. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target in the recombinant pEXPR clone will also be fused to a C-terminal S•TagTM coding sequence. The S•Tag sequence allows quantification of the fusion protein using the FRETWorks™ assay (Cat. No 70724). The tag can also be used for purification and detection. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.