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| | Anti-Cleaved Bad (Asp61) Rabbit pAb |
|  | Cat. No. AP1056 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids surrounding the Asp61 caspase cleavage site of human Bad | | Form: Liquid | | Formulation: In PBS, 0.05% BSA. | | Preservative: ≤0.1% sodium azide | | Positive Control: TNF-a-treated HeLa cells | | Comments: Recognizes the ~18 kDa cleaved Bad protein in TNF-a-treated HeLa cells. | | Ref.: Condorelli, F., et al. 2001.Mol Cell Biol. 9, 3025. Simakajornboon, N., et al. 2001.Brain Res. 1, 111. | | S: A09 |
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Detection of human cleaved bad (Asp61) by immunoblotting. Samples: whole cell lysate from untreated HeLa cells (lane A) and TNF-a-treated HeLa cells (10 ng/ml for 2 h). Primary antibody: Anti-Cleaved Bad (Asp61) Rabbit pAb (Cat. No. AP1056 ) (1:500). Detection: chemiluminescence.
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