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| | Anti-Cleaved ROCK-1 (Asp1113) Mouse mAb (154C1465) |
|  | Cat. No. PK1201 |
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| Host: Mouse | | Isotype: IgG1 | | Immunogen: a synthetic peptide (GNLPESRIEGWLS) corresponding to amino acids 1114-1126 of human ROCK-1 | | Form: Liquid | | Formulation: In PBS, 0.05% BSA. | | Preservative: ≤ 0.1% sodium azide | | Positive Control: Jurkat cells treated with camptothecin or anti-Fas | | Comments: Recognizes ~ 31 kDa cleaved ROCK-1 protein in apoptotic Jurkat cells. | | Ref.: Sebbagh M, et al. 2001. Nat Cell Biol. 3, 346. Amano, M. et al. 1996. J. Biol. Chem. 271, 20246. Nakagawa O., et al. 1996. FEBS Lett. 392, 189. |
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| 154C1465 | canine, human, monkey, mouse, rabbit, rat | IB
See Key |
Detection of human cleaved ROCK-1 by immunoblotting. Samples: whole cell lysate (10 µg/lane) from Jurkat cells treated with camptothecin (Lane 1) and treated with anti-Fas antibody (10 ng/ml 2 h, Lane 2). Primary antibody: Anti-Cleaved ROCK-1 (Asp1113) Mouse mAb (154C1465) (Cat. No. PK1201) (1 µg/ml). Detection: chemiluminescence.
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